PMID- 7877615
OWN - NLM
STAT- MEDLINE
DCOM- 19950404
LR  - 20081121
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 8
IP  - 11
DP  - 1994 Nov
TI  - Reconstitution of thyroid hormone receptor and retinoic acid receptor function in 
      the fission yeast Schizosaccharomyces pombe.
PG  - 1455-64
AB  - We report here a characterization of the thyroid hormone receptors (T3Rs), 
      retinoic acid receptors (RARs), and retinoid X receptors (RXRs) by reconstituting 
      their actions in the fission yeast Schizosaccharomyces pombe. S. pombe provide a 
      well defined and readily manipulated genetic background devoid of known 
      endogenous nuclear hormone receptors. All the receptors tested, when introduced 
      exogenously into S. pombe, induced high levels of reporter gene activation in 
      response to physiological concentrations of hormone ligand. In these properties, 
      the S. pombe system exhibits significant advantages over the previously employed 
      Saccharomyces cerevisiae system. Use of the S. pombe system permitted the 
      elucidation of previously undescribed differences in the DNA sequence recognition 
      properties of different isoforms of the RXR and RARs, and the identification of 
      apparently novel forms of response element for RXRs and RARs. Intriguingly, the 
      v-erb A allele of T3R, a transcriptional repressor in vertebrate cells, acts as a 
      transcriptional activator both in S. cerevisiae and in the evolutionarily highly 
      divergent S. pombe, underscoring the importance of cellular factors in the 
      regulation of receptor transcriptional activity.
FAU - Sande, S
AU  - Sande S
AD  - Department of Microbiology, University of California at Davis 95616.
FAU - Privalsky, M L
AU  - Privalsky ML
LA  - eng
GR  - 1-CA53394-03/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (Oncogene Proteins v-erbA)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Base Sequence
MH  - Cell Nucleus/metabolism
MH  - Genes/physiology
MH  - Genes, Reporter
MH  - Molecular Sequence Data
MH  - Oncogene Proteins v-erbA/pharmacology
MH  - Promoter Regions, Genetic
MH  - Receptors, Retinoic Acid/genetics/*metabolism
MH  - Receptors, Thyroid Hormone/genetics/*metabolism
MH  - Retinoid X Receptors
MH  - Schizosaccharomyces/genetics
MH  - Transcription Factors/genetics/metabolism/physiology
MH  - *Transcription, Genetic
MH  - Transformation, Genetic
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1210/mend.8.11.7877615 [doi]
PST - ppublish
SO  - Mol Endocrinol. 1994 Nov;8(11):1455-64. doi: 10.1210/mend.8.11.7877615.