PMID- 7879851
OWN - NLM
STAT- MEDLINE
DCOM- 19950331
LR  - 20190821
IS  - 0003-2654 (Print)
IS  - 0003-2654 (Linking)
VI  - 119
IP  - 12
DP  - 1994 Dec
TI  - Immunochemical detection of antibiotics and sulfonamides.
PG  - 2543-8
AB  - To control the maximum residue limits (MRLs) for residues of veterinary drugs in 
      food of animal origin, according to EU regulations, a broad spectrum of sensitive 
      analytical methods is required. One effective approach is the development of 
      immunoassays, particularly for screening purposes. Strategies for the production 
      of specific polyclonal and monoclonal antibodies against beta-lactams, 
      tetracyclines, streptomycin, chloramphenicol, sulfonamides and trimethoprim, are 
      outlined, as well as methods for the synthesis of the respective enzyme-labelled 
      antigens. The sensitivity and the specificity of the antibodies were 
      characterized, and the immunochemical test systems were designed as quantitative 
      routine tests (microtitre plate format) and as rapid qualitative tests 
      (membrane-based assay formats). The detection limits of the assays were found to 
      be well below the regulatory limits. The range of recovery, for the analysis of 
      artificially contaminated samples, was between 68 and 104%. In principle, the 
      enzyme immunoassays for antimicrobial drugs showed the advantage of sensitivity 
      and speed together with the simplicity of manipulations involved in the 
      procedure. However, because of the results of the specificity studies, as well as 
      the possibility of false positive results owing to unspecific inhibition of the 
      assay, confirmation of immunoassay results is still required for all legal and 
      statutory purposes.
FAU - Martlbauer, E
AU  - Martlbauer E
AD  - Institute for Hygiene and Technology of Food of Animal Origin, Veterinary 
      Faculty, University of Munich, Germany.
FAU - Usleber, E
AU  - Usleber E
FAU - Schneider, E
AU  - Schneider E
FAU - Dietrich, R
AU  - Dietrich R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Review
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antibodies)
RN  - 0 (Immunoglobulins)
RN  - 0 (Sulfonamides)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Antibodies
MH  - Cattle
MH  - Drug Residues/*analysis
MH  - Female
MH  - Immunoassay/methods
MH  - Immunoenzyme Techniques
MH  - Immunoglobulins
MH  - Milk/chemistry
MH  - Sensitivity and Specificity
MH  - Sulfonamides/*analysis
RF  - 40
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1039/an9941902543 [doi]
PST - ppublish
SO  - Analyst. 1994 Dec;119(12):2543-8. doi: 10.1039/an9941902543.