PMID- 7879885
OWN - NLM
STAT- MEDLINE
DCOM- 19950331
LR  - 20190821
IS  - 0003-2654 (Print)
IS  - 0003-2654 (Linking)
VI  - 119
IP  - 12
DP  - 1994 Dec
TI  - Particle beam liquid chromatography-mass spectrometry method with negative ion 
      chemical ionization for the confirmation of oxacillin, cloxacillin and 
      dicloxacillin residues in bovine muscle.
PG  - 2731-6
AB  - To ensure human food safety, the European Union has defined maximum residue 
      limits (MRLs) for veterinary drug residues in food products. Analytical methods 
      need to be developed to confirm the presence of drugs at the MRL level. A method 
      using particle beam liquid chromatography-mass spectrometry was developed for the 
      confirmation of oxacillin, cloxacillin and dicloxacillin in bovine muscle at the 
      maximum residue limit of 300 micrograms kg-1. Beta-lactams were extracted from 
      tissues with ethyl acetate under slightly acidic conditions and separated on a 
      C18 bonded silica column with a methanol-aqueous formic acid (2%) 
      solution-acetonitrile mobile phase. Negative ion chemical ionization with methane 
      as the reagent gas was used to identify the compounds. The specificity required 
      for a regulatory confirmation procedure was achieved by monitoring five fragment 
      ions for each compound (selected ion monitoring mode): m/z 183, 198, 213, 214 and 
      241 for oxacillin; m/z 196, 248, 250, 275 and 277 for cloxacillin; m/z 165, 230, 
      232, 274 and 276 for dicloxacillin.
FAU - Hurtaud, D
AU  - Hurtaud D
AD  - Ministere de l'Agriculture et de la Foret, Centre National d'Etudes Veterinaires 
      et Alimentaires, Eougeres, France.
FAU - Delepine, B
AU  - Delepine B
FAU - Sanders, P
AU  - Sanders P
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - COF19H7WBK (Dicloxacillin)
RN  - O6X5QGC2VB (Cloxacillin)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Chromatography, High Pressure Liquid/methods
MH  - Cloxacillin/*analysis
MH  - Dicloxacillin/*analysis
MH  - Drug Residues/*analysis
MH  - Flame Ionization/methods
MH  - Humans
MH  - Mass Spectrometry/methods
MH  - Meat/*analysis
MH  - Muscle, Skeletal/*chemistry
MH  - Oxacillin/*analysis
MH  - Safety
MH  - Sensitivity and Specificity
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1039/an9941902731 [doi]
PST - ppublish
SO  - Analyst. 1994 Dec;119(12):2731-6. doi: 10.1039/an9941902731.