PMID- 7880399
OWN - NLM
STAT- MEDLINE
DCOM- 19950413
LR  - 20190920
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 32
IP  - 3
DP  - 1994 Oct
TI  - Nuclear regulation of HLA class I genes in human trophoblasts.
PG  - 167-72
AB  - PROBLEM: Human trophoblast expression of class I human leukocyte antigen (HLA) 
      genes is unique in that there is no classical gene expression, but nonclassical 
      HLA-G is expressed and only by cytotrophoblast cells. This differential 
      expression of classical versus nonclassical class I genes suggests tissue 
      specific regulation. Recently, a negative regulatory element (NRE), 180 bp 5' to 
      transcription initiation was identified in a murine embryonal carcinoma cell line 
      that markedly inhibited class I gene expression (Flanagan et al., Proc Natl Acad 
      Sci USA 1991; 83:3145-3149). METHOD: Here we analyzed the human HLA-A2 gene for a 
      putative NRE sequence and determined whether such a sequence is capable of 
      binding to factors present in a variety of class I-null cell lines. RESULTS: 
      Sequence analysis revealed that the NRE for human HLA-A2 is identical to that for 
      mouse H-2Ld. Using gel shift assays with nuclear extracts (NE) from a variety of 
      cell types, we demonstrated specific binding to the HLA-A2 NRE sequence. The 
      choriocarcinoma cell lines JEG and BeWo and the F9 cells (all negative for 
      classical gene expression) contained this DNA binding factor(s). This binding 
      factor was not present in NE from lymphocytes or a variety of other cell lines 
      that were positive for classical gene expression. CONCLUSION: Human trophoblasts 
      appear to have a tissue specific nuclear binding factor that may down regulate 
      classical class I expression upon binding to the NRE sequence. The HLA-G gene 
      does not have this NRE region thus enabling its expression by these cells.
FAU - Chiang, M H
AU  - Chiang MH
AD  - Institute of Child Health and Human Development, California Pacific Medical 
      Center Research Institute, San Francisco 94115.
FAU - Main, E K
AU  - Main EK
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Base Sequence
MH  - Cell Nucleus/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Female
MH  - Gene Expression Regulation/immunology
MH  - HLA Antigens/*biosynthesis/*genetics
MH  - HLA-A2 Antigen/*biosynthesis/*genetics
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*biosynthesis/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Regulatory Sequences, Nucleic Acid
MH  - Trophoblasts/*immunology
MH  - Tumor Cells, Cultured
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1111/j.1600-0897.1994.tb01109.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1994 Oct;32(3):167-72. doi: 
      10.1111/j.1600-0897.1994.tb01109.x.