PMID- 7882576
OWN - NLM
STAT- MEDLINE
DCOM- 19950413
LR  - 20190825
IS  - 0305-1870 (Print)
IS  - 0305-1870 (Linking)
VI  - 21
IP  - 11
DP  - 1994 Nov
TI  - Enhancement of endothelium-dependent relaxation in the aorta from stroke-prone 
      spontaneously hypertensive rats at developmental stages of hypertension.
PG  - 857-63
AB  - 1. Endothelium-dependent relaxation (EDR) in aortic rings from young (8 weeks) 
      and adult (16 weeks and 20 weeks) stroke-prone spontaneously hypertensive rats 
      (SHRSP) was investigated in comparison with age-matched Wistar-Kyoto rats (WKY). 
      2. At 8 weeks, acetylcholine (3 x 10(-9)-10(-5) mol/L) and ionomycin (4 x 
      10(-8)-10(-6) mol/L)-induced EDR in SHRSP aortae was significantly enhanced 
      compared to that in WKY aortae. Mechanical denudation of the endothelium 
      completely abolished, and pretreatment of aortae with NG-monomethyl L-arginine (1 
      mmol/L), an inhibitor of nitric oxide formation, greatly reduced the relaxation 
      in both strains. Indomethacin (10(-5) mol/L), a cyclo-oxygenase inhibitor that 
      blocks the production of endothelium-derived contracting factors, did not 
      significantly alter the relaxation by acetylcholine at this age. There was no 
      difference in endothelium-independent relaxation of denuded aortae by sodium 
      nitroprusside (10(-9)-10(-6) mol/L) and 8-bromoguanosine 3', 5'-cyclic 
      monophosphate (10(-6)-10(-3) mol/L). 3. In adult SHRSP with established 
      hypertension, however, the acetylcholine (10(-8)-10(-5) mol/L)-induced relaxation 
      markedly diminished at any of the concentrations tested compared to that observed 
      in 8 weeks old SHRSP and WKY at 8-20 weeks of age. This finding differed from 
      other observations where the relaxation in SHRSP was impaired only at higher 
      concentrations of acetylcholine. Indomethacin pretreatment of aortae from 20 week 
      old SHRSP restored acetylcholine-induced EDR to a level comparable with that in 
      age-matched WKY.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Onda, T
AU  - Onda T
AD  - University of Shizuoka, School of Pharmaceutical Sciences, Japan.
FAU - Mashiko, S
AU  - Mashiko S
FAU - Hamano, M
AU  - Hamano M
FAU - Tomita, I
AU  - Tomita I
FAU - Tomita, T
AU  - Tomita T
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Clin Exp Pharmacol Physiol
JT  - Clinical and experimental pharmacology & physiology
JID - 0425076
RN  - 0 (Endothelins)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - N9YNS0M02X (Acetylcholine)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Aorta/drug effects/physiology/physiopathology
MH  - Cerebrovascular Disorders/*physiopathology
MH  - Dose-Response Relationship, Drug
MH  - Endothelins/*pharmacology
MH  - Hypertension/*physiopathology
MH  - Indomethacin/pharmacology
MH  - Muscle, Smooth, Vascular/*drug effects/physiology
MH  - Nitric Oxide/*pharmacology
MH  - Rats
MH  - Rats, Inbred SHR/*growth & development
MH  - Rats, Wistar
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1111/j.1440-1681.1994.tb02456.x [doi]
PST - ppublish
SO  - Clin Exp Pharmacol Physiol. 1994 Nov;21(11):857-63. doi: 
      10.1111/j.1440-1681.1994.tb02456.x.