PMID- 7884042
OWN - NLM
STAT- MEDLINE
DCOM- 19950411
LR  - 20071114
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 350
IP  - 2
DP  - 1994 Dec 8
TI  - Immunocytochemical localization of the beta 2 subunit of the gamma-aminobutyric 
      acidA receptor in the rat brain.
PG  - 260-71
AB  - An antiserum to the beta 2 subunit of the rat gamma-aminobutyric acid (GABAA) 
      receptor was prepared by immunizing a rabbit with a fusion protein expressed in 
      bacteria. The fusion protein had the large, intracellular loop expanding between 
      the putative M3 and M4 transmembrane domains of the beta 2 subunit fused to 
      staphylococcal protein A (SPA). The antiserum immunoprecipitated both the 
      solubilized and the affinity-purified GABAA receptors. The anti-beta 2 antibodies 
      were affinity purified on immobilized beta 2 intracellular loop peptide. The 
      antibodies recognized a 55-57 kDa peptide in immunoblots of either crude 
      membranes from rat cerebral cortex or affinity-purified GABAA receptors from 
      bovine cerebral cortex. Immunocytochemistry with the affinity-purified antibody 
      has revealed for the first time the localization of the beta 2 subunit in the rat 
      brain. A comparative study of the regional and cellular immunoreactivities of the 
      affinity-purified anti-beta 2 antibody and the monoclonal antibody 62-3G1 (which 
      recognizes both beta 2 and beta 3 subunits) is presented. The procedure described 
      for generating and preparing specific anti-beta 2 subunit antibodies that are 
      valuable for immunocytochemistry could be extended to other GABAA receptor 
      subunits.
FAU - Moreno, J I
AU  - Moreno JI
AD  - Division of Molecular Biology and Biochemistry, School of Biological Sciences, 
      University of Missouri-Kansas City 64110-2499.
FAU - Piva, M A
AU  - Piva MA
FAU - Miralles, C P
AU  - Miralles CP
FAU - De Blas, A L
AU  - De Blas AL
LA  - eng
GR  - NS17708/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, GABA-A)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Staphylococcal Protein A)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Specificity
MH  - Base Sequence
MH  - Brain Chemistry/*physiology
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Peptide Fragments/*analysis
MH  - Precipitin Tests
MH  - Rats
MH  - Receptors, GABA-A/*analysis
MH  - Recombinant Fusion Proteins/isolation & purification
MH  - Staphylococcal Protein A
EDAT- 1994/12/08 00:00
MHDA- 1994/12/08 00:01
CRDT- 1994/12/08 00:00
PHST- 1994/12/08 00:00 [pubmed]
PHST- 1994/12/08 00:01 [medline]
PHST- 1994/12/08 00:00 [entrez]
AID - 10.1002/cne.903500209 [doi]
PST - ppublish
SO  - J Comp Neurol. 1994 Dec 8;350(2):260-71. doi: 10.1002/cne.903500209.