PMID- 7884321
OWN - NLM
STAT- MEDLINE
DCOM- 19950413
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 57
IP  - 3
DP  - 1995 Mar
TI  - Differential regulation of fyn-associated protein tyrosine kinase activity by 
      macrophage colony-stimulating factor (M-CSF) and granulocyte-macrophage 
      colony-stimulating factor (GM-CSF).
PG  - 484-90
AB  - The proliferation and differentiation of macrophages are regulated by, among 
      others, GM-CSF and M-CSF. Treatment of bone marrow nonadherent (NA) cells with 
      M-CSF induced a greater percentage of NA cells into adherent cells, recognized as 
      monocyte/macrophages, than did GM-CSF. The effect of GM-CSF and M-CSF on the 
      activation of fyn kinase, a 59-kDa src family-related protein tyrosine kinase 
      (PTK), was studied. Control cultures of bone marrow NA cells expressed only 
      minimal levels of fyn kinase activity. Treatment of bone marrow NA cells with 
      M-CSF, but not GM-CSF, for 12 to 24 h greatly enhanced the levels of fyn kinase 
      activity. The effect of M-CSF on the activation of fyn kinase was further 
      investigated using bipotential adherent bone marrow-derived macrophages (BMDMs) 
      that coexpress receptors for both GM-CSF and M-CSF. BMDMs can be induced by 
      either growth factor to undergo extensive proliferation in vitro. Compared to 
      bone marrow NA cells, BMDMs displayed higher levels of basal fyn kinase activity, 
      which were similarly elevated by M-CSF but not GM-CSF treatment. The role of fyn 
      kinase in regulating cell adhesion was investigated by growing BMDMs in both 
      tissue culture and Teflon flasks. The growth of BMDMs was anchorage independent; 
      the majority of them continued to proliferate as cell suspension in Teflon 
      flasks. Whereas the levels of fyn kinase activity in adherent BMDMs grown in 
      tissue culture flasks increased steadily, those in BMDMs grown in Teflon flasks 
      diminished. To study the role of fyn kinase in growth regulation, BMDMs were 
      treated with c-fyn sense and antisense s-oligos. In the presence of c-fyn 
      antisense s-oligos, the proliferative response of BMDMs to M-CSF but not GM-CSF 
      was inhibited. In contrast, the proliferation of BMDM in response to either 
      GM-CSF or M-CSF was not influenced by c-fyn sense s-oligos. Collectively, our 
      data suggest that the activation of fyn kinase is closely associated with the 
      acquisition of adherent capacity in maturing macrophages.
FAU - Li, Y
AU  - Li Y
AD  - Department of Internal Medicine, Wayne State University School of Medicine, 
      Detroit, Michigan 48201.
FAU - Chen, B
AU  - Chen B
LA  - eng
GR  - AI23499/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 0 (Recombinant Proteins)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.2 (Fyn protein, mouse)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-fyn)
SB  - IM
GS  - c-fyn
MH  - Animals
MH  - Base Sequence
MH  - Bone Marrow Cells
MH  - Cell Adhesion
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - In Vitro Techniques
MH  - Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Macrophages/*enzymology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Molecular Sequence Data
MH  - Oligonucleotides, Antisense/pharmacology
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-fyn
MH  - Receptor, Macrophage Colony-Stimulating Factor/metabolism
MH  - Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Recombinant Proteins
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1002/jlb.57.3.484 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1995 Mar;57(3):484-90. doi: 10.1002/jlb.57.3.484.