PMID- 7884899
OWN - NLM
STAT- MEDLINE
DCOM- 19950410
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 69
IP  - 4
DP  - 1995 Apr
TI  - Fgf-8, activated by proviral insertion, cooperates with the Wnt-1 transgene in 
      murine mammary tumorigenesis.
PG  - 2501-7
AB  - We have used mouse mammary tumor virus (MMTV) infection of Wnt-1 transgenic mice 
      to accelerate mammary tumorigenesis and to molecularly tag insertionally 
      activated proto-oncogenes that cooperate oncogenically with Wnt-1 (G. M. 
      Shackleford, C. A. MacArthur, H. C. Kwan, and H. E. Varmus, Proc. Natl. Acad. 
      Sci. USA 90:740-744, 1993). Here we report the identification and 
      characterization of a 31-kb genomic locus that contains clonal MMTV integrations 
      in 8 of 80 mammary tumors from MMTV-infected Wnt-1 transgenic mice. Two genes 
      were identified within this locus, one of which was transcriptionally activated 
      by MMTV insertions. This activated gene is identical to androgen-induced growth 
      factor (AIGF/Fgf-8) (A. Tanaka, K. Miyamoto, N. Minamino, M. Takeda, B. Sato, H. 
      Matsuo, and K. Matsumoto, Proc. Natl. Acad. Sci. USA 89:8928-8932, 1992), the 
      eighth member of the fibroblast growth factor (FGF) family. Transcriptional 
      activation of Fgf-8 was found in all tumors with MMTV insertions in this locus. 
      Fgf-8 mRNA was absent in normal mammary glands and was detected only in adult 
      testis and ovary and in midgestational embryos. The sequences of Fgf-8 genomic 
      and cDNA clones revealed five coding exons, in contrast to the three coding exons 
      found in other FGF genes. cDNAs encoding three isoforms of the FGF-8 protein were 
      isolated. The three corresponding mRNAs resulted from the alternative use of two 
      5' splice sites and two 3' splice sites for the second and third exons, 
      respectively. These results implicate Fgf-8 as the third FGF gene found to 
      cooperate with Wnt-1 in MMTV-induced murine mammary tumorigenesis, suggesting 
      that FGFs and Wnts are strong collaborators in this process.
FAU - MacArthur, C A
AU  - MacArthur CA
AD  - Department of Pediatrics, University of Southern California School of Medicine, 
      Los Angeles.
FAU - Shankar, D B
AU  - Shankar DB
FAU - Shackleford, G M
AU  - Shackleford GM
LA  - eng
SI  - GENBANK/U18673
GR  - CA58412/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA Primers)
RN  - 0 (Fgf8 protein, mouse)
RN  - 0 (Growth Substances)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Wnt Proteins)
RN  - 0 (Wnt1 Protein)
RN  - 0 (Wnt1 protein, mouse)
RN  - 0 (Zebrafish Proteins)
RN  - 148997-75-5 (Fibroblast Growth Factor 8)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
GS  - Fgf-8
GS  - Wnt-1
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Exons
MH  - Female
MH  - Fibroblast Growth Factor 8
MH  - *Fibroblast Growth Factors
MH  - Growth Substances/*genetics
MH  - Mammary Neoplasms, Experimental/*genetics/virology
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*genetics
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proviruses/*genetics
MH  - RNA Splicing
MH  - Virus Integration
MH  - Wnt Proteins
MH  - Wnt1 Protein
MH  - *Zebrafish Proteins
PMC - PMC188926
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
PMCR- 1995/04/01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
PHST- 1995/04/01 00:00 [pmc-release]
AID - 10.1128/JVI.69.4.2501-2507.1995 [doi]
PST - ppublish
SO  - J Virol. 1995 Apr;69(4):2501-7. doi: 10.1128/JVI.69.4.2501-2507.1995.