PMID- 7885731
OWN - NLM
STAT- MEDLINE
DCOM- 19950407
LR  - 20190818
IS  - 0031-1820 (Print)
IS  - 0031-1820 (Linking)
VI  - 110 ( Pt 2)
DP  - 1995 Feb
TI  - Differentiation between human and animal strains of Cryptosporidium parvum using 
      isoenzyme typing.
PG  - 129-32
AB  - Isoenzyme typing was used to study a number of oocyst isolates of Cryptosporidium 
      parvum from different geographical locations and of human or animal origin. All 
      isolates showed identical enzyme motility when glucose phosphate isomerase (GPI; 
      23 isolates tested) or lactate dehydrogenases (LDH; 20 isolates tested) was 
      assayed. However, two isoenzyme forms were observed with phosphoglucomutase (PGM; 
      9 animal isolates showed one form, while 8/9 human isolates showed a second form) 
      and hexokinase (HK; 4 human isolates showed one form and 6 animal isolates showed 
      a second form). Thus, PGM and HK each exhibit 2 isoenzymes corresponding to 2 
      parasite populations associated with separate hosts. The data from this study, 
      plus supportive evidence obtained by different methods and by independent 
      researchers, lend support to the hypothesis that separate cycles of transmission 
      of C. parvum may exist within human and animal hosts.
FAU - Awad-el-Kariem, F M
AU  - Awad-el-Kariem FM
AD  - Department of Clinical Sciences, London School of Hygiene and Tropical Medicine.
FAU - Robinson, H A
AU  - Robinson HA
FAU - Dyson, D A
AU  - Dyson DA
FAU - Evans, D
AU  - Evans D
FAU - Wright, S
AU  - Wright S
FAU - Fox, M T
AU  - Fox MT
FAU - McDonald, V
AU  - McDonald V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Parasitology
JT  - Parasitology
JID - 0401121
RN  - 0 (Isoenzymes)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
RN  - EC 5.4.2.2 (Phosphoglucomutase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cryptosporidiosis/epidemiology/parasitology
MH  - Cryptosporidium parvum/*classification/*enzymology
MH  - Feces/parasitology
MH  - Glucose-6-Phosphate Isomerase/isolation & purification
MH  - Hexokinase/isolation & purification
MH  - Humans
MH  - Isoenzymes/isolation & purification
MH  - L-Lactate Dehydrogenase/isolation & purification
MH  - Phosphoglucomutase/isolation & purification
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 10.1017/s0031182000063885 [doi]
PST - ppublish
SO  - Parasitology. 1995 Feb;110 ( Pt 2):129-32. doi: 10.1017/s0031182000063885.