PMID- 7886686
OWN - NLM
STAT- MEDLINE
DCOM- 19950410
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 96
IP  - 2
DP  - 1995 Feb 6
TI  - Dietary vitamin A enhances sensitivity of the local lymph node assay.
PG  - 157-63
AB  - Murine assays such as the mouse ear swelling test (MEST) and the local lymph node 
      assay (LLNA) are popular alternatives to guinea pig models for the identification 
      of contact sensitizers, yet there has been concern over the effectiveness of 
      these assays to detect weak and moderate sensitizers. Much work has been done to 
      improve the sensitivity of the MEST, including the addition of a vitamin A 
      acetate (VAA) enriched diet, which increases its sensitivity. Vitamin A acetate 
      has been reported to increase the numbers of Langerhans cells (antigen presenting 
      cells) in the skin, which could in turn enhance the cellular immune response. 
      Because the LLNA relies on tritiated-thymidine incorporation by proliferating T 
      cells during the induction phase, we have studied the potential of the VAA diet 
      to enhance sensitivity of the LLNA. Results indicate that the VAA enriched diet 
      significantly increased the LLNA sensitivity to formalin, eugenol, 
      glutaraldehyde, trimellitic anhydride, and an azo dye at concentrations where no 
      proliferation was observed in mice maintained on the standard diet. Maintenance 
      on a VAA diet for 3 weeks prior to initiating the sensitization procedure was 
      optimal. Thus, incorporation of a VAA diet improves the sensitivity of the LLNA 
      as a quick, objective, and relatively inexpensive screen for detecting moderate 
      and weak contact sensitizers.
FAU - Sailstad, D M
AU  - Sailstad DM
AD  - ManTech Environmental Technology, Inc., Research Triangle Park, NC 27709.
FAU - Krishnan, S D
AU  - Krishnan SD
FAU - Tepper, J S
AU  - Tepper JS
FAU - Doerfler, D L
AU  - Doerfler DL
FAU - Selgrade, M K
AU  - Selgrade MK
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 11103-57-4 (Vitamin A)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Dermatitis, Allergic Contact/*immunology
MH  - Female
MH  - Immunologic Tests
MH  - Lymph Nodes/*drug effects/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Vitamin A/*pharmacology
EDAT- 1995/02/06 00:00
MHDA- 1995/02/06 00:01
CRDT- 1995/02/06 00:00
PHST- 1995/02/06 00:00 [pubmed]
PHST- 1995/02/06 00:01 [medline]
PHST- 1995/02/06 00:00 [entrez]
AID - 0300483X9402957V [pii]
AID - 10.1016/0300-483x(94)02957-v [doi]
PST - ppublish
SO  - Toxicology. 1995 Feb 6;96(2):157-63. doi: 10.1016/0300-483x(94)02957-v.