PMID- 7888210
OWN - NLM
STAT- MEDLINE
DCOM- 19950417
LR  - 20071114
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 10
IP  - 11
DP  - 1994 Nov
TI  - Molecular epidemiology of HTLV type I in Japan: evidence for two distinct 
      ancestral lineages with a particular geographical distribution.
PG  - 1557-66
AB  - Japan is one of the highest endemic areas of the world for human T cell 
      leukemia-lymphoma virus type I (HTLV-I). To gain new insight as to the origin of 
      this virus in Japan and especially in the southern islands of the archipelago, we 
      investigated the long terminal repeat (LTR) of 67 newly isolated HTLV-I proviral 
      DNAs from peripheral blood mononuclear cells of HTLV-I-infected individuals for 
      their restriction fragment length polymorphism (RFLP). The specimens were from 
      Japanese living in different geographical areas (Hokkaido, Honshu, Kyushu, or the 
      Ryukyu Islands) of Japan (59 cases) or Americans of Japanese ancestry living in 
      Hawaii (8 cases). The analysis of the results, together with data for the 19 
      previously published LTR sequences, demonstrated the existence of 2 subtypes of 
      HTLV-I in Japan. The first, which we propose to name Japanese subtype (previously 
      named subtype III), is more frequent (67 of 86: 78%) than the second, the 
      cosmopolitan subtype (previously named subtype II) (19 of 86: 22%). In parallel, 
      a fragment of 413 base pairs of the U3/R region (nucleotide 22 to 434) was cloned 
      and sequenced from 10 of the new Japanese samples. The alignment of these 
      sequences and their comparison and phylogenetic analysis with previously 
      published LTR HTLV-I sequences, demonstrated clearly the existence of the two 
      distinct molecular subtypes of HTLV-I in Japan, diverging in this LTR region by 
      about 1.6%. Furthermore, the study of the geographical distribution of the 2 
      subtypes among the 80 samples from patients whose place of residence in Japan was 
      known showed an uneven distribution. While the Japanese subtype was present in 
      all parts of Japan, the cosmopolitan subtype seemed to cluster in the southern 
      islands of the archipelago (i.e., Kyushu and the Ryukyu Islands) as well as in 
      immigrants from those areas who had lived in Hawaii for decades. These new 
      molecular data raise questions and suggest hypotheses, discussed here, concerning 
      the origin and means of dissemination of these human retrovirus subtypes in 
      Japan.
FAU - Vidal, A U
AU  - Vidal AU
AD  - Unite d'Epidemiologie des Virus Oncogenes, Institut Pasteur, Paris, France.
FAU - Gessain, A
AU  - Gessain A
FAU - Yoshida, M
AU  - Yoshida M
FAU - Mahieux, R
AU  - Mahieux R
FAU - Nishioka, K
AU  - Nishioka K
FAU - Tekaia, F
AU  - Tekaia F
FAU - Rosen, L
AU  - Rosen L
FAU - De The, G
AU  - De The G
LA  - eng
GR  - RR03061/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Consensus Sequence
MH  - DNA Primers/genetics
MH  - DNA, Viral/genetics
MH  - Epidemiologic Methods
MH  - HTLV-I Infections/epidemiology/*virology
MH  - Human T-lymphotropic virus 1/classification/*genetics/isolation & purification
MH  - Humans
MH  - Japan/epidemiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymorphism, Restriction Fragment Length
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1089/aid.1994.10.1557 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 1994 Nov;10(11):1557-66. doi: 
      10.1089/aid.1994.10.1557.