PMID- 7889866
OWN - NLM
STAT- MEDLINE
DCOM- 19950419
LR  - 20181113
IS  - 0091-6765 (Print)
IS  - 0091-6765 (Linking)
VI  - 102 Suppl 6
IP  - Suppl 6
DP  - 1994 Oct
TI  - Arylamines suppress their own activation and that of nitroarenes in V79 Chinese 
      hamster cells by competing for acetyltransferases.
PG  - 95-7
AB  - The effect of 2-aminofluorene (2-AF) on the toxicity of 2-aminoanthracene (2-AA) 
      and 1,6-dinitropyrene (1,6-DNP) was studied in N-acetyltransferase-proficient 
      V79-NHr1A2 cells genetically engineered for the expression of cytochrome P4501A2, 
      and in wild-type V79-NH cells. 2-AA inhibited the growth of V79-NHr1A2 cells and 
      induced the formation of micronuclei at concentrations of 0.1 to 1.0 microM, but 
      was virtually without toxic effects at a concentration of 10 microM. Addition of 
      2-AF protected against the cytotoxic and genotoxic effects elicited by low 
      concentrations of 2-AA. Half-maximum protection was observed at 0.2 to 0.5 microM 
      2-AF. The arylamine also prevented the cytotoxicity caused by 1,6-DNP in V79-NH 
      cells and completely suppressed the formation of 1-acetylamino-6-nitropyrene from 
      1,6-DNP in these cells. The results indicate that arylamines and related 
      N-hydroxyarylamines are substrates for the same acetyltransferase in V79-NH 
      cells. In consequence, arylamines are capable of suppressing the activation of 
      their proximate cytotoxic and genotoxic products in these cells and, presumably, 
      in vivo.
FAU - Kiefer, F
AU  - Kiefer F
AD  - Institut fur Toxikologie, GSF-Forschungszentrum fur Umwelt und Gesundheit, 
      Neuherberg, Germany.
FAU - Cumpelik, O
AU  - Cumpelik O
FAU - Reen, R
AU  - Reen R
FAU - Doehmer, J
AU  - Doehmer J
FAU - Wiebel, F J
AU  - Wiebel FJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Anthracenes)
RN  - 0 (Carcinogens)
RN  - 0 (Fluorenes)
RN  - 0 (Mutagens)
RN  - 0 (Pyrenes)
RN  - 3A69OS195N (2-aminofluorene)
RN  - 66Q2ZUF83N (1,6-dinitropyrene)
RN  - 8240818JGU (2-anthramine)
RN  - EC 2.3.1.- (Acetyltransferases)
SB  - IM
MH  - Acetyltransferases/*metabolism
MH  - Animals
MH  - Anthracenes/*toxicity
MH  - Binding, Competitive
MH  - CHO Cells
MH  - Carcinogens/*toxicity
MH  - Cricetinae
MH  - Drug Antagonism
MH  - Fluorenes/*pharmacology
MH  - Mutagens/*toxicity
MH  - Pyrenes/*toxicity
PMC - PMC1566860
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
PMCR- 1994/10/01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
PHST- 1994/10/01 00:00 [pmc-release]
AID - 10.1289/ehp.94102s695 [doi]
PST - ppublish
SO  - Environ Health Perspect. 1994 Oct;102 Suppl 6(Suppl 6):95-7. doi: 
      10.1289/ehp.94102s695.