PMID- 7890279
OWN - NLM
STAT- MEDLINE
DCOM- 19950420
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 26
IP  - 3
DP  - 1995 Mar
TI  - Intratumoral activation of CD8-positive cytotoxic lymphocytes in acquired 
      immunodeficiency syndrome lymphomas.
PG  - 284-90
AB  - The incidence of lymphomas is unusually high in human immunodeficiency virus 
      (HIV)-infected patients. Because cytotoxic T lymphocytes (CTL) represent a major 
      mechanism of the antitumoral immune response in immunocompetent individuals, we 
      asked whether intratumoral activation of CTL was impaired in acquired immune 
      deficiency syndrome (AIDS) lymphomas. Immunohistochemical experiments showed that 
      in AIDS lymphomas intratumoral CD8-positive T lymphocytes accumulated and 
      expressed the TIA-1 antigen, a marker of cytotoxic cells. Flow cytometry studies 
      and in situ hybridization of lymphomatous tissue confirmed the differentiation of 
      CD8-positive cells in cytotoxic cells and their activation, as assessed by their 
      expression of CD38 and human leukocyte antigen (HLA) DR markers as well as the 
      perforin and granzyme B genes, which code for two molecules involved in target 
      cell killing. On average, perforin-producing cells were as numerous in AIDS 
      lymphomas (5,647 +/- 2,655 cells/cm2) as in lymphomas from immunocompetent 
      individuals (3,294 +/- 1,544 cells/cm2). The density of activated CD8-positive 
      cells in the 22 AIDS lymphomas tested was not correlated with peripheral 
      CD4-positive cell counts. These results suggest that in AIDS lymphomas the steps 
      of differentiation and activation of cytotoxic CD8-positive cells are not altered 
      by immune deficiency and that they can take place through pathways relatively 
      independent of CD4-positive T lymphocytes. Thus, other mechanisms of immune 
      deficiency should account for the increased frequency of lymphomas in patients 
      with AIDS.
FAU - Devergne, O
AU  - Devergne O
AD  - Departement d'Hematologie, Hopital de la Pitie, Paris, France.
FAU - Raphael, M
AU  - Raphael M
FAU - Autran, B
AU  - Autran B
FAU - Leger-Ravet, M B
AU  - Leger-Ravet MB
FAU - Coumbaras, J
AU  - Coumbaras J
FAU - Crevon, M C
AU  - Crevon MC
FAU - Galanaud, P
AU  - Galanaud P
FAU - Emilie, D
AU  - Emilie D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Poly(A)-Binding Proteins)
RN  - 0 (Pore Forming Cytotoxic Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (T-Cell Intracellular Antigen-1)
RN  - 0 (TIA1 protein, human)
RN  - 126465-35-8 (Perforin)
RN  - EC 3.4.21.- (GZMB protein, human)
RN  - EC 3.4.21.- (Granzymes)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Adult
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Child, Preschool
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Granzymes
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - *Lymphocyte Activation
MH  - Lymphoma, AIDS-Related/genetics/*immunology/metabolism
MH  - Membrane Glycoproteins/genetics
MH  - *Membrane Proteins
MH  - Perforin
MH  - Poly(A)-Binding Proteins
MH  - Pore Forming Cytotoxic Proteins
MH  - *Proteins
MH  - RNA-Binding Proteins/analysis
MH  - Serine Endopeptidases/genetics
MH  - T-Cell Intracellular Antigen-1
MH  - Tumor Cells, Cultured
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 0046-8177(95)90059-4 [pii]
AID - 10.1016/0046-8177(95)90059-4 [doi]
PST - ppublish
SO  - Hum Pathol. 1995 Mar;26(3):284-90. doi: 10.1016/0046-8177(95)90059-4.