PMID- 7892188
OWN - NLM
STAT- MEDLINE
DCOM- 19950420
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 92
IP  - 6
DP  - 1995 Mar 14
TI  - Hydrodynamic studies of a complex between the Fc fragment of human IgE and a 
      soluble fragment of the Fc epsilon RI alpha chain.
PG  - 1841-5
AB  - The interaction between immunoglobulin E (IgE) and its high-affinity receptor Fc 
      epsilon RI is central to allergic disease. The binding site for Fc epsilon RI 
      lies in the third constant region domain of the epsilon heavy chain of IgE (C 
      epsilon 3). Identical epitopes on the two C epsilon 3 domains in the IgE-Fc are 
      predicted to be on opposite sides of the structure, and therefore each could bind 
      independently to a receptor molecule. Titrations, however, reveal that the IgE-Fc 
      forms an equimolar complex with a soluble fragment of the Fc epsilon RI alpha 
      chain (sFc epsilon RI alpha), and the molecular weight of the complex, as 
      determined by sedimentation equilibrium, confirms this stoichiometry. The 
      measured sedimentation coefficients of the two ligands are in good agreement with 
      computed values for a compact IgE-Fc and an elongated sFc epsilon RI alpha 
      structure. The calculated sedimentation coefficients for possible models of a 1:1 
      complex lead to exclusion of all highly extended geometries for the complex. 
      Possible explanations for the paradoxical stoichiometry of the IgE-Fc/sFc epsilon 
      RI alpha complex, in terms of the curved shape of IgE, a conformational change in 
      IgE when the receptor binds, and steric interference between two molecules of Fc 
      epsilon RI binding to identical sites, are discussed.
FAU - Keown, M B
AU  - Keown MB
AD  - Randall Institute, King's College London, United Kingdom.
FAU - Ghirlando, R
AU  - Ghirlando R
FAU - Young, R J
AU  - Young RJ
FAU - Beavil, A J
AU  - Beavil AJ
FAU - Owens, R J
AU  - Owens RJ
FAU - Perkins, S J
AU  - Perkins SJ
FAU - Sutton, B J
AU  - Sutton BJ
FAU - Gould, H J
AU  - Gould HJ
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Carbohydrate Sequence
MH  - Humans
MH  - Immunoglobulin E/*chemistry/isolation & purification/metabolism
MH  - Immunoglobulin Fc Fragments/*chemistry/isolation & purification/metabolism
MH  - Kinetics
MH  - Macromolecular Substances
MH  - Models, Structural
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Receptors, IgE/*chemistry/isolation & purification/metabolism
PMC - PMC42378
EDAT- 1995/03/14 00:00
MHDA- 1995/03/14 00:01
PMCR- 1995/09/14
CRDT- 1995/03/14 00:00
PHST- 1995/03/14 00:00 [pubmed]
PHST- 1995/03/14 00:01 [medline]
PHST- 1995/03/14 00:00 [entrez]
PHST- 1995/09/14 00:00 [pmc-release]
AID - 10.1073/pnas.92.6.1841 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):1841-5. doi: 10.1073/pnas.92.6.1841.