PMID- 7892260
OWN - NLM
STAT- MEDLINE
DCOM- 19950420
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 92
IP  - 6
DP  - 1995 Mar 14
TI  - Insertional mutagenesis identifies a member of the Wnt gene family as a candidate 
      oncogene in the mammary epithelium of int-2/Fgf-3 transgenic mice.
PG  - 2268-72
AB  - Transgenic mice harboring the int-2/Fgf-3 protooncogene under transcriptional 
      control of the mouse mammary tumor virus (MMTV) promoter/enhancer exhibit a 
      dramatic, benign hyperplasia of the mammary gland. In one int-2 transgenic line 
      (TG.NX), this growth disturbance is evoked by pregnancy and regresses after 
      parturition. Regression of hyperplastic mammary epithelium is less complete after 
      successive pregnancies, and, within 10 months, most TG.NX mice stochastically 
      develop mammary carcinomas that are transplantable in virgin, syngeneic mice. To 
      identify genes that cooperate with int-2 in cell transformation, we infected 
      TG.NX transgenic mice with MMTV. In a cohort of 14 animals, most mammary tumors 
      represented clonal or oligoclonal outgrowths harboring one to five proviral MMTV 
      integrants. Eight of 35 (23%) MMTV+ tumors exhibited proviral insertion at the 
      Wnt-1 locus. No provirus was detected at the int-2, int-3, or Wnt-3 loci. By 
      Southern analysis, two tumors had proviral insertions at the same genomic 
      location, which was mapped to chromosome 15. Cloning of this int locus identified 
      an additional member of the Wnt gene family. The predicted 389-amino acid protein 
      is most closely related to zebrafish Wnt-10a (58% amino acid identity over 362 
      residues) and, based on homology analysis, was designated Wnt-10b. This newly 
      discovered Wnt family member was expressed in the embryo and mammary gland of 
      virgin but not pregnant mice and represents a candidate collaborating oncogene of 
      int-2/Fgf-3 in the mammary epithelium.
FAU - Lee, F S
AU  - Lee FS
AD  - Department of Genetics, Harvard Medical School, Boston, MA 02115.
FAU - Lane, T F
AU  - Lane TF
FAU - Kuo, A
AU  - Kuo A
FAU - Shackleford, G M
AU  - Shackleford GM
FAU - Leder, P
AU  - Leder P
LA  - eng
SI  - GENBANK/U20658
GR  - 5 P2 CA58203-03/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Primers)
RN  - 0 (Fgf3 protein, mouse)
RN  - 0 (Fibroblast Growth Factor 3)
RN  - 0 (Genetic Markers)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (fgf3 protein, zebrafish)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
GS  - Fgf-3
GS  - Wnt
GS  - int-2
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - DNA Primers
MH  - Epithelium/metabolism
MH  - Female
MH  - Fibroblast Growth Factor 3
MH  - Fibroblast Growth Factors/*biosynthesis/genetics
MH  - Genetic Markers
MH  - Mammary Glands, Animal/*metabolism
MH  - Mammary Neoplasms, Experimental/classification/*genetics/pathology/virology
MH  - Mammary Tumor Virus, Mouse/genetics/pathogenicity
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Mutagenesis, Insertional
MH  - Neoplasm Proteins/*biosynthesis/genetics
MH  - *Oncogenes
MH  - Polymerase Chain Reaction
MH  - Protein-Tyrosine Kinases/biosynthesis
MH  - Proto-Oncogene Proteins/*biosynthesis/genetics
MH  - *Zebrafish Proteins
PMC - PMC42465
EDAT- 1995/03/14 00:00
MHDA- 1995/03/14 00:01
PMCR- 1995/09/14
CRDT- 1995/03/14 00:00
PHST- 1995/03/14 00:00 [pubmed]
PHST- 1995/03/14 00:01 [medline]
PHST- 1995/03/14 00:00 [entrez]
PHST- 1995/09/14 00:00 [pmc-release]
AID - 10.1073/pnas.92.6.2268 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2268-72. doi: 10.1073/pnas.92.6.2268.