PMID- 789348
OWN - NLM
STAT- MEDLINE
DCOM- 19761223
LR  - 20210526
IS  - 0021-9193 (Print)
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Linking)
VI  - 128
IP  - 1
DP  - 1976 Oct
TI  - Molecular cloning of an Escherichia coli plasmid determinant than encodes for the 
      production of heat-stable enterotoxin.
PG  - 463-72
AB  - A conjugative plasmid, ESF0041 was isolated from an enterotoxigenic strain of 
      Escherichia coli from calves. ESF0041 was found to be 65 x 10(6) daltons in mass 
      of a member of the F incompatibility complex. Acquisition of ESF0041 by E. coli 
      K-12 was invariably associated with the capacity to produce heat-stable (ST) 
      enterotoxin. ESF0041 and pSC101 deoxyribonucleic acids were cleaved with EcoRI, 
      and the fragments were ligated with polynucleotide ligase. Transformation of E. 
      coli K-12 with the ligation mixture led to the isolation of an ST+ clone. Further 
      analysis of the plasmid deoxyribonucleic acid from this clone showed that a 
      structural gene(s) associated with ST biosynthesis had been isolated as a 5.7 x 
      10(6)-dalton ESF0041 fragment in pSC101. In turn, 5.7 x 10(6)-dalton fragment was 
      ligated to a multicopy COLE1 derivative, RSF2124, so that toxin synthesis was 
      amplified about threefold.
FAU - So, M
AU  - So M
FAU - Boyer, H W
AU  - Boyer HW
FAU - Betlach, M
AU  - Betlach M
FAU - Falkow, S
AU  - Falkow S
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Enterotoxins)
SB  - IM
MH  - DNA Replication
MH  - Enterotoxins/*biosynthesis
MH  - *Escherichia coli/metabolism
MH  - *Extrachromosomal Inheritance
MH  - *Genes
MH  - *Plasmids
MH  - Transformation, Genetic
PMC - PMC232874
EDAT- 1976/10/01 00:00
MHDA- 1976/10/01 00:01
PMCR- 1976/10/01
CRDT- 1976/10/01 00:00
PHST- 1976/10/01 00:00 [pubmed]
PHST- 1976/10/01 00:01 [medline]
PHST- 1976/10/01 00:00 [entrez]
PHST- 1976/10/01 00:00 [pmc-release]
AID - 10.1128/jb.128.1.463-472.1976 [doi]
PST - ppublish
SO  - J Bacteriol. 1976 Oct;128(1):463-72. doi: 10.1128/jb.128.1.463-472.1976.