PMID- 7894241
OWN - NLM
STAT- MEDLINE
DCOM- 19950427
LR  - 20191101
IS  - 0940-2993 (Print)
IS  - 0940-2993 (Linking)
VI  - 46
IP  - 4-5
DP  - 1994 Oct
TI  - Necklace-like detachment of endothelial cell layer from arterial wall under 
      low-calcium condition.
PG  - 307-13
AB  - The diversified morphological manifestations in various tissues and organs 
      obtained by administration of differing amounts of calcium chelating agents were 
      reported in previous papers (Yamaguchi et al 1981 a & b; 1982; 1990; 1993). In 
      our recent research described here, administration of a moderate dose of Na2EDTA 
      over the short term demonstrated necklace-like detachment from the arterial wall 
      without disruption of the endothelial cell chain. Intercellular spaces in the 
      media just beneath the detached endothelial cell layer was stained strongly with 
      colloidal iron staining. Electron microscopic observation revealed that the 
      detached endothelial cells showed a lot of elongated anchoring villi from the 
      basal surface, usually seen at the luminal surface, adhered to the degenerative 
      and thin flattened internal elastic lamellae. The alteration of the colloidal 
      iron staining of the vascular wall under the low-calcium condition is suggested 
      to be induced by loosening of the molecular structure of glycosaminoglycans 
      (GAGs) as well as glycoproteins (GPs), comprising the important component of the 
      intercellular matrix and elastic lamellae, which would induce a change in their 
      pasty or viscous character. This would be an accelerative factor for detachment 
      of endothelial cells. Moreover, the lack of the waving of the internal elastic 
      lamellae, trapping of endothelial cytoplasmic processes among them, would play 
      the decisive role in the total detachment of the endothelial cell layer. On the 
      other hand, the low-calcium condition did not adversely influence the joining of 
      endothelial cells. The pathognomatic mechanism will be discussed, with a 
      comparison made to the angiolytic changes provoked by a large amount of Na2EDTA.
FAU - Yamaguchi, H
AU  - Yamaguchi H
AD  - Department of Pathology, School of Medicine, Keio University, Japan.
FAU - Morisada, M
AU  - Morisada M
FAU - Kaku, H
AU  - Kaku H
FAU - Onodera, T
AU  - Onodera T
FAU - Kurokawa, R
AU  - Kurokawa R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Exp Toxicol Pathol
JT  - Experimental and toxicologic pathology : official journal of the Gesellschaft fur 
      Toxikologische Pathologie
JID - 9208920
RN  - 0 (Glycosaminoglycans)
RN  - 9007-58-3 (Elastin)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*deficiency
MH  - Cell Adhesion/drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Edetic Acid/*toxicity
MH  - Elastin/analysis
MH  - Endothelium, Vascular/chemistry/*cytology/ultrastructure
MH  - Female
MH  - Glycosaminoglycans/analysis
MH  - Guinea Pigs
MH  - Male
MH  - Mesenteric Arteries/cytology/drug effects/ultrastructure
MH  - Microscopy, Electron
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1016/s0940-2993(11)80107-7 [doi]
PST - ppublish
SO  - Exp Toxicol Pathol. 1994 Oct;46(4-5):307-13. doi: 10.1016/s0940-2993(11)80107-7.