PMID- 7895677
OWN - NLM
STAT- MEDLINE
DCOM- 19950425
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 136
IP  - 4
DP  - 1995 Apr
TI  - Differential expression and regulation of cytokine genes in the mouse uterus.
PG  - 1666-73
AB  - The present investigation examined the differential expression of cytokine genes 
      in vivo and in vitro in the mouse uterus and their regulation by ovarian steroid 
      hormones. The expression of interleukin-1 beta (IL-1 beta), IL-1 alpha, IL-6, and 
      tumor necrosis factor-alpha (TNF alpha) genes was examined in the mouse uterus as 
      well as in freshly isolated or cultured epithelial cells by Northern blot and in 
      situ hybridization. In the day 1 pregnant (D1 = vaginal plug) uterus, the levels 
      of IL-1 alpha and IL-1 beta messenger RNAs (mRNAs) were abundant, whereas those 
      of TNF alpha and IL-6 were at the limits of detection. Freshly isolated D1 
      uterine epithelial cell preparations contained higher levels of IL-1 alpha and 
      IL-1 beta mRNAs than those observed in the D1 whole uterus, whereas TNF alpha and 
      IL-6 mRNA levels were consistently low. In contrast, D1 epithelial cells showed 
      decreased levels of IL-1 beta mRNA after 1 day of culture, whereas the levels of 
      IL-1 alpha and IL-6 mRNAs increased under similar conditions. The levels of IL-1, 
      IL-6, and TNF alpha mRNAs were at the limits of detection in the D4 whole uterus 
      or freshly isolated diestrous epithelial cells. However, IL-1 alpha and IL-6 mRNA 
      levels in diestrous epithelial cells, like those in D1 epithelial cells, 
      increased in culture. In contrast, IL-1 beta and TNF alpha mRNA levels remained 
      low in cultured epithelial cells. In situ hybridization was used to examine the 
      cell type-specific expression of IL-1 alpha or IL-6 mRNA in uterine sections and 
      cultured cells. Although hybridization signals for IL-1 alpha mRNA were detected 
      in uterine epithelial cells on D1 of pregnancy, IL-6 mRNA could not be detected. 
      IL-1 alpha and IL-6 mRNAs could not be detected in freshly isolated diestrous 
      epithelial cells, although a majority of the epithelial cells showed 
      hybridization signals for these mRNAs after 2 or 4 days of culture. The effects 
      of steroid hormones on uterine cytokine gene expression were examined by Northern 
      blot and in situ hybridization. In adult ovariectomized mice, an injection of 17 
      beta-estradiol (E2), progesterone (P4), or a combination of E2 and P4 had little 
      or no apparent effect on these cytokine mRNA levels. The results establish that 
      uterine epithelial cells on D1 of pregnancy exhibit heightened expression of IL-1 
      alpha in culture. In contrast, these cells express little or no IL-6 mRNA in 
      vivo, but show heightened expression in culture. These results suggest that an 
      apparent loss of repression of these uterine genes occurs in culture. 
      Furthermore, E2 and/or P4 treatments appear to have little or no effect on 
      uterine cytokine mRNA levels in adult ovariectomized mice.
FAU - Kover, K
AU  - Kover K
AD  - Department of Physiology, Ralph L. Smith Research Center, University of Kansas 
      Medical Center, Kansas City 66160-7338.
FAU - Liang, L
AU  - Liang L
FAU - Andrews, G K
AU  - Andrews GK
FAU - Dey, S K
AU  - Dey SK
LA  - eng
GR  - CA-61262/CA/NCI NIH HHS/United States
GR  - HD-12304/HD/NICHD NIH HHS/United States
GR  - HD-29968/HD/NICHD NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cytokines/*genetics
MH  - Estradiol/pharmacology
MH  - Female
MH  - *Gene Expression Regulation
MH  - In Situ Hybridization
MH  - Interleukin-1/genetics
MH  - Interleukin-6/genetics
MH  - Mice
MH  - Ovariectomy
MH  - Progesterone/pharmacology
MH  - RNA, Messenger/metabolism
MH  - Tumor Necrosis Factor-alpha/genetics
MH  - Uterus/*metabolism
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 10.1210/endo.136.4.7895677 [doi]
PST - ppublish
SO  - Endocrinology. 1995 Apr;136(4):1666-73. doi: 10.1210/endo.136.4.7895677.