PMID- 7896273
OWN - NLM
STAT- MEDLINE
DCOM- 19950421
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 24
IP  - 1
DP  - 1994 Nov 1
TI  - Direct mapping of seven genes encoding human type 2 cystatins to a single site 
      located at 20p11.2.
PG  - 172-5
AB  - The human type 2 cystatins are encoded by a multigene family of eight to nine 
      members. Five functional genes (CST1-CST5) and two pseudogenes (CSTP1 and CSTP2) 
      have previously been cloned. We have developed a panel of short genomic fragments 
      that serve as gene-specific probes for these seven genes under hybridization 
      conditions used for Southern blots or fluorescence in situ hybridization (FISH). 
      Analysis of BssHII-digested genomic DNA using these probes was consistent with 
      association of all seven genes with a 905-kb fragment, suggesting physical 
      clustering at a single site. The cluster was assigned to chromosome 20 by 
      screening a somatic cell hybrid panel with an STS for CST2. This assignment was 
      confirmed for each gene by FISH using repeat-free gene-specific genomic probes 
      ranging in size from 2.3 to 0.8 kb. All the loci mapped to 20p11.2.
FAU - Dickinson, D P
AU  - Dickinson DP
AD  - Department of Basic Sciences, University of Texas-Houston Health Science Center 
      77225.
FAU - Zhao, Y
AU  - Zhao Y
FAU - Thiesse, M
AU  - Thiesse M
FAU - Siciliano, M J
AU  - Siciliano MJ
LA  - eng
GR  - CA34936/CA/NCI NIH HHS/United States
GR  - CA55164/CA/NCI NIH HHS/United States
GR  - DE08902/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Cystatins)
RN  - 0 (DNA Primers)
SB  - IM
GS  - CST
GS  - CSTP1
GS  - CSTP2
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 20
MH  - Cricetinae
MH  - Cystatins/*genetics
MH  - DNA Primers
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - S0888-7543(84)71595-3 [pii]
AID - 10.1006/geno.1994.1595 [doi]
PST - ppublish
SO  - Genomics. 1994 Nov 1;24(1):172-5. doi: 10.1006/geno.1994.1595.