PMID- 7898031
OWN - NLM
STAT- MEDLINE
DCOM- 19950427
LR  - 20061115
IS  - 0023-6764 (Print)
IS  - 0023-6764 (Linking)
VI  - 44
IP  - 6
DP  - 1994 Dec
TI  - Transcription of multiple exogenous mouse mammary tumor viruses in a single 
      mammary tumor of an SHN mouse.
PG  - 584-9
AB  - The SHN mouse strain, established in Japan independently from other mammary 
      tumor-prone strains, is known to have a very high incidence of early-onset 
      mammary tumors. In this study, we demonstrate that exogenous mouse mammary tumor 
      virus (MMTV) plays a crucial role in early-onset mammary tumorigenesis in this 
      strain. We subsequently isolated MMTV RNA from a mammary tumor of an SHN female 
      mouse, reverse transcribed it, and amplified a 1.2-kb coding region of the long 
      terminal repeat (LTR) by use of the polymerase chain reaction. The products were 
      then subcloned into a pUC18 vector. Six clones were obtained, and their 
      nucleotide sequences were determined. Nucleotide sequences of the six cloned LTRs 
      indicated that two MMTVs are transcribed in the tumor. Both of them had point 
      mutations and were different from known LTR sequences. Both LTRs had a potential 
      open reading frame encoding 320 amino acids. The amino acid variabilities were 
      predominantly between positions 280 and 320 codons when compared with deduced 
      open reading frames. In contrast, only a few point mutations were detected in the 
      regulatory area that contains five glucocorticoid elements, the nuclear factor-1 
      binding sites, the octamer consensus sequence, and the TATA box. Because point 
      mutations occur more predominantly between positions 280 and 320, it is possible 
      that mutations in this area endow MMTV with beneficial functions for propagation 
      through mutational selection during tumorigenesis.
FAU - Koizumi, A
AU  - Koizumi A
AD  - Department of Hygiene, Akita University School of Medicine, Japan.
FAU - Tsukada, M
AU  - Tsukada M
FAU - Sugawara, K
AU  - Sugawara K
FAU - Kusano, T
AU  - Kusano T
LA  - eng
SI  - GENBANK/S75270
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Anim Sci
JT  - Laboratory animal science
JID - 1266503
RN  - 0 (Codon)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Codon
MH  - Female
MH  - Male
MH  - Mammary Neoplasms, Experimental/*virology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Point Mutation
MH  - Polymerase Chain Reaction
MH  - RNA, Viral/chemistry/genetics
MH  - Repetitive Sequences, Nucleic Acid
MH  - *Transcription, Genetic
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
PST - ppublish
SO  - Lab Anim Sci. 1994 Dec;44(6):584-9.