PMID- 7902522
OWN - NLM
STAT- MEDLINE
DCOM- 19931229
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 53
IP  - 23
DP  - 1993
TI  - Gx/z and Gi2 transducer proteins on mu/delta opioid-mediated supraspinal 
      antinociception.
PG  - PL381-6
AB  - Intracerebroventricular (i.c.v.) administration of immune sera raised against Gi2 
      alpha subunits to mice, significantly reduced the supraspinal antinociceptive 
      effect of opioids when evaluated 24 h later in the tail-flick test. Antisera 
      directed against Gi1 alpha subunits did not modify this opioid activity. In mice 
      injected with sera anti-Gx/z alpha, the mu-preferential agonists, DAMGO and 
      morphine, and the endogenous mu/delta opioid peptide beta-endorphin-(1-31) 
      displayed a reduced antinociceptive activity, whereas, the potency of the 
      delta-selective agonists DPDPE and [D-Ala2]Deltorphin II, was not altered. This 
      reduction was present for 3 to 7 days and returned to the control values after 10 
      days. Anti-Gi2 alpha and anti-Gx/z alpha, but not anti-Gi1 alpha, reduced the 
      specific binding of [3H]DAMGO to the opioid receptor in PAG. These results 
      suggest the ability of the mu receptor to interact in vivo with different classes 
      of G transducer proteins (Gx/z/Gi2) to produce an effect. This work also 
      indicates a functional role of the pertussis toxin insensitive Gx/z protein, on 
      the mu-mediated (but not delta-mediated) supraspinal antinociception in mice.
FAU - Sanchez-Blazquez, P
AU  - Sanchez-Blazquez P
AD  - Neurofarmacologia, Instituto Cajal, C.S.I.C., Madrid, Spain.
FAU - Juarros, J L
AU  - Juarros JL
FAU - Martinez-Pena, Y
AU  - Martinez-Pena Y
FAU - Castro, M A
AU  - Castro MA
FAU - Garzon, J
AU  - Garzon J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Enkephalins)
RN  - 0 (Immune Sera)
RN  - 0 (Narcotic Antagonists)
RN  - 0 (Receptors, Opioid, delta)
RN  - 0 (Receptors, Opioid, mu)
RN  - 100929-53-1 (Enkephalin, Ala(2)-MePhe(4)-Gly(5)-)
RN  - 58822-25-6 (Enkephalin, Leucine)
RN  - 88373-73-3 (Enkephalin, D-Penicillamine (2,5)-)
RN  - 89352-67-0 (N,N-diallyl-tyrosyl-alpha-aminoisobutyric acid-phenylalanyl-leucine)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Analgesics, Opioid/*pharmacology
MH  - Analysis of Variance
MH  - Animals
MH  - Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
MH  - Enkephalin, D-Penicillamine (2,5)-
MH  - Enkephalin, Leucine/analogs & derivatives/pharmacology
MH  - Enkephalins/pharmacology
MH  - GTP-Binding Proteins/immunology/*physiology
MH  - Immune Sera/immunology/pharmacology
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Narcotic Antagonists/pharmacology
MH  - Receptors, Opioid, delta/*drug effects
MH  - Receptors, Opioid, mu/*drug effects
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 0024-3205(93)90166-Z [pii]
AID - 10.1016/0024-3205(93)90166-z [doi]
PST - ppublish
SO  - Life Sci. 1993;53(23):PL381-6. doi: 10.1016/0024-3205(93)90166-z.