PMID- 7904747
OWN - NLM
STAT- MEDLINE
DCOM- 19940222
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 21
IP  - 25
DP  - 1993 Dec 25
TI  - Correlation between the presence of a self-splicing intron in the 25S rDNA of 
      C.albicans and strains susceptibility to 5-fluorocytosine.
PG  - 6020-7
AB  - Candida albicans presents a well characterized EcoRI RFLP pattern of intensely 
      staining bands. One of these bands, the dimorphic 3.7/4.2 kbp fragment shown to 
      originate from the ribosomal RNA-encoding regions (rDNA), has been used by 
      several investigators to subdivide C. albicans strains in two distinct subtypes. 
      In the present manuscript, we report that an epidemiological study of 120 
      C.albicans strains revealed a significant correlation between these subtypes and 
      susceptibility to 5-fluorocytosine, an antifungal agent extensively used for 
      biotyping C.albicans. The 4.2 kbp strains being generally more susceptible than 
      their counterparts to this agent and one of its metabolic by-product, 
      5-fluorouracil. A 379 nucleotides insertion in the 25S rRNA-encoding gene of 4.2 
      kbp type strains was shown to be responsible for the 3.7/4.2 size difference. 
      This intervening sequence is typical of a group I intron by its site of 
      insertion, its predicted secondary structure, and its self-splicing capability. 
      Assuming there is a genuine causal relationship between presence of the intron 
      and resistance to 5-fluorocytosine, one possible mechanism suggests that 
      inhibition of self-splicing by the insertion of 5-fluorouracil residues in the 
      25S rRNA precursor might be responsible for the higher susceptibility of 4.2 kbp 
      type strains.
FAU - Mercure, S
AU  - Mercure S
AD  - Departement de Microbiologie et Immunologie, Faculte de Medecine, Universite de 
      Montreal, Quebec, Canada.
FAU - Montplaisir, S
AU  - Montplaisir S
FAU - Lemay, G
AU  - Lemay G
LA  - eng
SI  - GENBANK/X74272
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA, Fungal)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Ribosomal)
RN  - 130527-23-0 (RNA, ribosomal, 25S)
RN  - D83282DT06 (Flucytosine)
SB  - IM
MH  - Base Sequence
MH  - Candida albicans/drug effects/*genetics
MH  - DNA, Fungal
MH  - DNA, Ribosomal/*genetics
MH  - Flucytosine/*pharmacology
MH  - *Introns
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Polymorphism, Restriction Fragment Length
MH  - *RNA Splicing
MH  - RNA, Fungal
MH  - RNA, Ribosomal/chemistry/*genetics
MH  - Repetitive Sequences, Nucleic Acid
PMC - PMC310489
EDAT- 1993/12/25 00:00
MHDA- 1993/12/25 00:01
CRDT- 1993/12/25 00:00
PHST- 1993/12/25 00:00 [pubmed]
PHST- 1993/12/25 00:01 [medline]
PHST- 1993/12/25 00:00 [entrez]
AID - 10.1093/nar/21.25.6020 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1993 Dec 25;21(25):6020-7. doi: 10.1093/nar/21.25.6020.