PMID- 7906601
OWN - NLM
STAT- MEDLINE
DCOM- 19940325
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 629
IP  - 2
DP  - 1993 Dec 3
TI  - The expression of amyloid beta protein precursor protects nerve cells from 
      beta-amyloid and glutamate toxicity and alters their interaction with the 
      extracellular matrix.
PG  - 275-82
AB  - The biological function of amyloid beta protein precursor (ABPP) in nerve cells 
      is examined by transfecting it into the B103 clonal nerve cell line which makes 
      no ABPP and asking if a variety of biological activities are altered. Although 
      ABPP is expressed by the transfected cells at high levels, there are no 
      detectable differences between the ABPP negative clones and the clones expressing 
      ABPP with respect to growth rate in high serum medium, dibutyryl-cyclic 
      AMP-induced differentiation, or morphology on plastic culture dishes. There are, 
      however, significant differences in a number of properties between the two groups 
      of cell lines. Cells expressing ABPP adhere more rapidly and have an enhanced 
      rate of neurite outgrowth on collagen substrata. They also grow more rapidly in 
      low serum medium. Finally, the expression of ABPP weakly but significantly 
      protects the nerve cells from amyloid beta protein and glutamate toxicity.
FAU - Schubert, D
AU  - Schubert D
AD  - Salk Institute for Biological Studies, San Diego, CA 92186-5800.
FAU - Behl, C
AU  - Behl C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Glutamates)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Amyloid beta-Peptides/metabolism/*toxicity
MH  - Amyloid beta-Protein Precursor/*biosynthesis/physiology
MH  - Blotting, Northern
MH  - Bucladesine/pharmacology
MH  - Cell Adhesion/drug effects
MH  - Cell Line
MH  - Clone Cells
MH  - Collagen/metabolism
MH  - Culture Media, Serum-Free
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Extracellular Matrix/*metabolism
MH  - Glutamates/metabolism/*toxicity
MH  - Glutamic Acid
MH  - Humans
MH  - Neurites/drug effects/ultrastructure
MH  - Plasmids
MH  - Transfection
EDAT- 1993/12/03 00:00
MHDA- 1993/12/03 00:01
CRDT- 1993/12/03 00:00
PHST- 1993/12/03 00:00 [pubmed]
PHST- 1993/12/03 00:01 [medline]
PHST- 1993/12/03 00:00 [entrez]
AID - 0006-8993(93)91331-L [pii]
AID - 10.1016/0006-8993(93)91331-l [doi]
PST - ppublish
SO  - Brain Res. 1993 Dec 3;629(2):275-82. doi: 10.1016/0006-8993(93)91331-l.