PMID- 7908510
OWN - NLM
STAT- MEDLINE
DCOM- 19940504
LR  - 20190920
IS  - 0166-3542 (Print)
IS  - 0166-3542 (Linking)
VI  - 23
IP  - 2
DP  - 1994 Feb
TI  - Mechanism of inhibitory effect of dextran sulfate and heparin on human T-cell 
      lymphotropic virus type I (HTLV-I)-induced syncytium formation in vitro: role of 
      cell-to-cell contact.
PG  - 143-59
AB  - Cell-to-cell contact is usually essential for syncytium formation by 
      HTLV-I-infected cell lines. The present study was undertaken to determine the 
      inhibitory effect of polyanionic compounds, dextran sulfate and heparin, on 
      HTLV-I-induced syncytium formation, as demonstrated by the fusion of 
      HTLV-I-infected cells with target cells. These two compounds almost completely 
      blocked syncytium formation in the early phase of the reaction at a concentration 
      of 125 micrograms/ml, but dextran, as a control, did not inhibit it at 
      concentrations up to 625 micrograms/ml. 50% inhibition of syncytium formation was 
      detected at a concentration of 2 micrograms/ml of dextran sulfate 5000, 3 
      micrograms/ml of dextran sulfate 8000 and 8 micrograms/ml of heparin. The binding 
      of radiolabeled HTLV-I-infected cells (HCT-1) to the target cells was inhibited 
      by addition of dextran sulfate and heparin, and the inhibitory effects were 
      concentration-dependent. No marked changes were detected in the expression of 
      adhesion molecules on the virus-infected cells and target cells, and in the 
      expression of envelope proteins on the virus-infected cells after exposing them 
      to the polyanionic compounds. These results suggest that the blocking of 
      cell-to-cell contact by polyanionic compounds, probably independent of surface 
      adhesion molecules, is important for their inhibitory effect on HTLV-I-induced 
      syncytium formation.
FAU - Ida, H
AU  - Ida H
AD  - First Department of Internal Medicine, Nagasaki University School of Medicine, 
      Japan.
FAU - Kurata, A
AU  - Kurata A
FAU - Eguchi, K
AU  - Eguchi K
FAU - Yamashita, I
AU  - Yamashita I
FAU - Nakashima, M
AU  - Nakashima M
FAU - Sakai, M
AU  - Sakai M
FAU - Kawabe, Y
AU  - Kawabe Y
FAU - Nakamura, T
AU  - Nakamura T
FAU - Nagataki, S
AU  - Nagataki S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Antiviral Res
JT  - Antiviral research
JID - 8109699
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Gene Products, env)
RN  - 0 (Gene Products, gag)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (HTLV-I Antigens)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (gp46 protein, Human T-cell leukemia virus type I)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 4C905MSK4W (Hexadimethrine Bromide)
RN  - 9005-49-6 (Heparin)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Cell Adhesion/drug effects
MH  - Cell Adhesion Molecules/analysis
MH  - Cell Communication/*drug effects/physiology
MH  - Cell Fusion/drug effects/physiology
MH  - Cell Line, Transformed
MH  - Dextran Sulfate/*pharmacology
MH  - Gene Products, env/analysis
MH  - Gene Products, gag/analysis
MH  - Giant Cells/*drug effects
MH  - HTLV-I Antibodies/blood
MH  - HTLV-I Antigens/analysis
MH  - Heparin/*pharmacology
MH  - Hexadimethrine Bromide/pharmacology
MH  - Human T-lymphotropic virus 1/drug effects/*physiology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1
MH  - Leukemia-Lymphoma, Adult T-Cell/immunology
MH  - Paraparesis, Tropical Spastic/immunology
MH  - Retroviridae Proteins, Oncogenic/analysis
MH  - Tumor Cells, Cultured
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - 0166-3542(94)90041-8 [pii]
AID - 10.1016/0166-3542(94)90041-8 [doi]
PST - ppublish
SO  - Antiviral Res. 1994 Feb;23(2):143-59. doi: 10.1016/0166-3542(94)90041-8.