PMID- 7910873
OWN - NLM
STAT- MEDLINE
DCOM- 19940627
LR  - 20071114
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 70
IP  - 5
DP  - 1994 May
TI  - Cytokine expression, upregulation of intercellular adhesion molecule-1, and 
      leukocyte infiltration in experimental tubulointerstitial nephritis.
PG  - 631-8
AB  - BACKGROUND: Cytokines are intercellular polypeptide messengers that mediate 
      immune and inflammatory responses. The temporal profile of interleukin-1 beta 
      (IL-1 beta), IL-6, tumor necrosis factor alpha (TNF-alpha), and monocyte 
      chemotactic protein 1 (MCP-1) expression was examined in anti-tubular basement 
      membrane (TBM) antibody-associated tubulointerstitial nephritis (TIN). 
      EXPERIMENTAL DESIGN: TIN was induced by immunization of Brown Norway rats with 
      bovine cortical TBM, whereas control rats received ovalbumin. Whole kidney RNA 
      was assessed with the RNase protection assay 3, 7, 8, 9, 10, 12, and 14 days 
      after immunization. Cytokine mRNA expression was correlated with TNF-alpha 
      bioactivity, renal intercellular adhesion molecule-1 expression, and 
      CD18-positive leukocyte infiltration by immunohistochemistry. RESULTS: Increased 
      IL-1 beta, TNF-alpha, and MCP-1 mRNA relative to glyceraldehyde-3-phosphate 
      dehydrogenase appeared on day 7 when TIN involved 10 to 40% of the cortex, and 
      peaked rapidly on day 8 when there was 60 to 80% cortical involvement (at which 
      time 75 to 80% of the infiltrating cells were neutrophils). The increase in 
      TNF-alpha mRNA correlated with increased bioactivity. The influx of mononuclear 
      cells on day 8 was preceded by the expression of MCP-1 mRNA. The infiltrating 
      leukocytes expressed the leukocyte beta 2-integrin (CD18) and were found in areas 
      with increased intercellular adhesion molecule-1 expression. The mRNAs for IL-1 
      beta, TNF-alpha, and MCP-1 were undetectable by day 10 (at which time 95% of the 
      infiltrating cells were mononuclear). An increase in IL-1 receptor antagonist 
      mRNA paralleled those of IL-1 beta. The expression of IL-6 mRNA was similar to 
      that for IL-1, except that it disappeared by day 9. CONCLUSIONS: There is a 
      temporal association in the expression of IL-1 beta, TNF alpha, MCP-1, and IL-6 
      with the upregulation of intercellular adhesion molecule-1 and leukocyte 
      infiltration within the tubulointerstitium in anti-TBM antibody-associated TIN. 
      The narrow window of time through which these cytokines are expressed and the 
      coincidence of their peak expression on day 8 suggest complex cytokine 
      interactions in the pathogenesis of anti-TBM antibody TIN.
FAU - Tang, W W
AU  - Tang WW
AD  - Department of Immunology, Scripps Research Institute, La Jolla, California.
FAU - Feng, L
AU  - Feng L
FAU - Mathison, J C
AU  - Mathison JC
FAU - Wilson, C B
AU  - Wilson CB
LA  - eng
GR  - DK20043/DK/NIDDK NIH HHS/United States
GR  - DK40251/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Antibodies)
RN  - 0 (Antisense Elements (Genetics))
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Antisense Elements (Genetics)
MH  - Base Sequence
MH  - Basement Membrane/immunology
MH  - Cattle
MH  - Cell Adhesion Molecules/*biosynthesis
MH  - Cytokines/*biosynthesis
MH  - DNA Primers
MH  - *Gene Expression
MH  - Gene Expression Regulation
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/analysis/biosynthesis
MH  - Immunization
MH  - Intercellular Adhesion Molecule-1
MH  - Interleukin-1/biosynthesis
MH  - Kidney Cortex/metabolism/pathology
MH  - Kidney Tubules/*immunology
MH  - Leukocytes/metabolism/*pathology
MH  - Molecular Sequence Data
MH  - Nephritis, Interstitial/immunology/*metabolism/*pathology
MH  - Polymerase Chain Reaction
MH  - RNA Probes
MH  - RNA, Messenger/analysis/*biosynthesis/metabolism
MH  - Rats
MH  - Rats, Inbred BN
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1994 May;70(5):631-8.