PMID- 7912704
OWN - NLM
STAT- MEDLINE
DCOM- 19940729
LR  - 20061115
IS  - 0263-6352 (Print)
IS  - 0263-6352 (Linking)
VI  - 12
IP  - 3
DP  - 1994 Mar
TI  - Dde I restriction fragment length polymorphism of the alpha 2-adrenoceptor gene 
      does not correlate with blood pressure in the F2 generation obtained from 
      crossing stroke-prone spontaneously hypertensive rats and Wistar-Kyoto rats.
PG  - 235-8
AB  - OBJECTIVE: A pathogenetic role of altered alpha 2-adrenoceptors in essential 
      hypertension has been suggested, based on studies in humans and animals. To 
      examine the role of the alpha 2-adrenoceptor in genetically hypertensive rats, we 
      compared the alpha 2-adrenoceptor genes of stroke-prone spontaneously 
      hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats by restriction fragment 
      length polymorphism analysis using human alpha 2-adrenoceptor probes (alpha 
      2-C10) and Dde I restriction endonuclease, and conducted a genetic cosegregation 
      study. METHOD: Five female WKY rats were bred with five male SHRSP. Eight pairs 
      of F1 rats were mated in brother-sister pairs to yield an F2 population of 84 
      rats. Systolic blood pressure was determined by tail-cuff sphygmomanometry. 
      Direct arterial blood pressure was taken under ether anaesthesia just before the 
      rats were killed. Southern blots were performed using alpha 2C10 as a probe and 
      the DNA from the F2 generation. RESULTS: A restriction fragment length 
      polymorphism of the SHRSP allele of a 1.6-kb fragment and a WKY rat allele of a 
      0.9-kb fragment with a common band of 1.3 kb in SHRSP and WKY rats was found, as 
      reported previously. The distribution of the genotype based on restriction 
      fragment length polymorphism conformed to a 1:2:1 ratio in F2 rats, as expected 
      for a Mendelian trait. There was no significant difference in the blood pressure 
      of F2 rats with respect to alpha 2-adrenoceptor genotype. CONCLUSION: This study 
      demonstrated that the alpha 2-adrenoceptor gene restriction fragment length 
      polymorphism distribution is a Mendelian trait in the F2 rats of crossed SHRSP 
      and WKY rats, but failed to show genetic cosegregation of this restriction 
      fragment length polymorphism with blood pressure in this generation.
FAU - Kobayashi, S
AU  - Kobayashi S
AD  - Second Department of Internal Medicine, Yokohama City University, School of 
      Medicine, Japan.
FAU - Umemura, S
AU  - Umemura S
FAU - Hirawa, N
AU  - Hirawa N
FAU - Iwamoto, T
AU  - Iwamoto T
FAU - Yamaguchi, S
AU  - Yamaguchi S
FAU - Tamura, K
AU  - Tamura K
FAU - Takasaki, I
AU  - Takasaki I
FAU - Ishii, M
AU  - Ishii M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Hypertens
JT  - Journal of hypertension
JID - 8306882
RN  - 0 (Receptors, Adrenergic, alpha-2)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.21.- (endodeoxyribonuclease DdeI)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Blood Pressure/*genetics/physiology
MH  - Cerebrovascular Disorders/genetics
MH  - Crosses, Genetic
MH  - DNA/genetics
MH  - Deoxyribonucleases, Type II Site-Specific
MH  - Female
MH  - Hypertension/*genetics/physiopathology
MH  - Male
MH  - *Polymorphism, Restriction Fragment Length
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Receptors, Adrenergic, alpha-2/*genetics/physiology
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
PST - ppublish
SO  - J Hypertens. 1994 Mar;12(3):235-8.