PMID- 7913418
OWN - NLM
STAT- MEDLINE
DCOM- 19940812
LR  - 20190825
IS  - 0009-2797 (Print)
IS  - 0009-2797 (Linking)
VI  - 92
IP  - 1-3
DP  - 1994 Jun
TI  - Human phenol sulfotransferases: chiral substrates and expression in Hep G2 cells.
PG  - 47-55
AB  - Enzymatic sulfation of chiral phenolic ethanolamine drugs, e.g. beta-agonists, 
      has been shown to be stereoselective in humans. The reaction appears to be 
      specific for the monoamine (M) form of the phenol sulfotransferases (PSTs). In 
      further studies of the stereochemistry of this reaction, we have found the 
      hepatoblastoma-derived cell line Hep G2 to be an excellent human model. These 
      cells contain the M form PST in quantities exceeding those of human liver by 
      about 4-fold. Thus, sulfate conjugates of the beta-agonist drugs can easily be 
      synthesized for subsequent structural and enzyme kinetic studies. Although less 
      abundant, the phenol (P) form PST as well as dehydroepiandrosterone 
      sulfotransferase are also expressed in the Hep G2 cells.
FAU - Walle, T
AU  - Walle T
AD  - Department of Pharmacology, Medical University of South Carolina, Charleston 
      29425.
FAU - Walle, U K
AU  - Walle UK
FAU - Shwed, J A
AU  - Shwed JA
FAU - Thornburg, K R
AU  - Thornburg KR
FAU - Mathis, C E
AU  - Mathis CE
FAU - Pesola, G R
AU  - Pesola GR
LA  - eng
GR  - GM46000/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - Ireland
TA  - Chem Biol Interact
JT  - Chemico-biological interactions
JID - 0227276
RN  - 0 (Adrenergic beta-Agonists)
RN  - EC 2.8.2.- (Sulfotransferases)
RN  - EC 2.8.2.- (dehydroepiandrosterone sulfotransferase)
RN  - EC 2.8.2.1 (Arylsulfotransferase)
SB  - IM
MH  - Adrenergic beta-Agonists/*metabolism
MH  - Arylsulfotransferase/biosynthesis/chemistry/*metabolism
MH  - Hepatoblastoma
MH  - Humans
MH  - Liver/enzymology
MH  - Liver Neoplasms
MH  - Stereoisomerism
MH  - Sulfotransferases/biosynthesis
MH  - Tumor Cells, Cultured
RF  - 26
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 0009-2797(94)90052-3 [pii]
AID - 10.1016/0009-2797(94)90052-3 [doi]
PST - ppublish
SO  - Chem Biol Interact. 1994 Jun;92(1-3):47-55. doi: 10.1016/0009-2797(94)90052-3.