PMID- 7913961
OWN - NLM
STAT- MEDLINE
DCOM- 19940901
LR  - 20111117
IS  - 0022-3492 (Print)
IS  - 0022-3492 (Linking)
VI  - 65
IP  - 5
DP  - 1994 May
TI  - Unique intronic variations of HLA-DQ beta gene in early-onset periodontitis.
PG  - 379-86
AB  - Human leukocyte antigen (HLA) class II beta chain plays an important role in the 
      recognition of foreign antigens in immune reactions. Different forms of immune 
      reaction may be concerned with initiation and progression of infectious diseases 
      such as periodontitis. In this study we examined the frequency of HLA class II 
      serotype and the variation of HLA class II beta gene in periodontitis patients. 
      HLA serotypic frequencies in 70 Japanese patients with periodontitis and 26 
      individuals with periodontal health were examined. No HLA serotype specific to 
      any type of periodontitis was observed. In order to detect differences among some 
      HLA serotypes, restriction fragment length polymorphism (RFLP) analysis was 
      undertaken with cDNA probes for HLA-DR beta and HLA-DQ beta genes in 20 subjects 
      (15 patients and 5 healthy individuals). Atypical BamHI and EcoRI restriction 
      sites were found in the HLA-DQ beta gene from 3 patients with early-onset 
      periodontitis. In addition to these 20 subjects, an additional 80 subjects (40 
      patients and 40 healthy individuals) were screened for the atypical BamHI 
      restriction site using the polymerase chain reaction method. It was detected in 7 
      patients with early-onset periodontitis, 1 patient with adult periodontitis, and 
      3 healthy subjects. No clinical differences except age were found between 
      patients with this gene variation and other patients. Interestingly, all 3 
      healthy subjects with this gene variation were from subjects whose family members 
      developed early-onset periodontitis with the gene variation. Atypical BamHI and 
      EcoRI restriction sites and 41-nt repeated sequence were found in the intron 
      before the third exon of HLA-DQB gene. These results suggest that these intronic 
      gene variations may be useful as gene markers for a subpopulation of early-onset 
      periodontitis and might affect immune reactions such as antigen recognition.
FAU - Takashiba, S
AU  - Takashiba S
AD  - Department of Periodontology and Endodontology, Okayama University Dental School, 
      Japan.
FAU - Noji, S
AU  - Noji S
FAU - Nishimura, F
AU  - Nishimura F
FAU - Ohyama, H
AU  - Ohyama H
FAU - Kurihara, H
AU  - Kurihara H
FAU - Nomura, Y
AU  - Nomura Y
FAU - Taniguchi, S
AU  - Taniguchi S
FAU - Murayama, Y
AU  - Murayama Y
LA  - eng
SI  - GENBANK/S73071
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (DNA, Complementary)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQbeta antigen)
RN  - 0 (HLA-DR Antigens)
RN  - EC 3.1.21.- (Deoxyribonuclease BamHI)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aggressive Periodontitis/*genetics/immunology
MH  - Base Sequence
MH  - Chi-Square Distribution
MH  - DNA, Complementary
MH  - Deoxyribonuclease BamHI
MH  - Female
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DQ beta-Chains
MH  - HLA-DR Antigens/genetics
MH  - Humans
MH  - Introns
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Periodontitis/genetics/immunology
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 10.1902/jop.1994.65.5.379 [doi]
PST - ppublish
SO  - J Periodontol. 1994 May;65(5):379-86. doi: 10.1902/jop.1994.65.5.379.