PMID- 7915160
OWN - NLM
STAT- MEDLINE
DCOM- 19940928
LR  - 20240109
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 6
IP  - 7
DP  - 1994 Jul
TI  - Oat phytochrome A mRNA degradation appears to occur via two distinct pathways.
PG  - 1007-19
AB  - We have identified possible mechanisms for the degradation of oat phytochrome A 
      (PHYA) mRNA. The majority of PHYA mRNA molecules appeared to be degraded prior to 
      removal of the poly(A) tail, a pathway that differs from that reported for the 
      degradation of other eukaryotic mRNAs. Polyadenylated PHYA mRNA contained a 
      pattern of putative degradation products that is consistent with a 5'-->3' 
      exoribonuclease, although the participation of a stochastic endoribonuclease 
      cannot be excluded. The poly(A) tail of PHYA mRNA was heterogeneous in size and 
      ranged from approximately 14 to 220 nucleotides. Early PHYA mRNA degradation 
      events did not appear to involve site-specific endoribonucleases. Approximately 
      25% of the apparently full-length PHYA mRNA was poly(A) deficient. Oat H4 
      histone, beta-tubulin, and actin mRNA populations had lower amounts of apparently 
      full-length mRNAs that were poly(A) deficient. Degradation of the 
      poly(A)-deficient PHYA mRNA, a second pathway, appeared to be initiated by a 
      3'-->5' exoribonucleolytic removal of the poly(A) tail followed by both 5'-->3' 
      and 3'-->5' exoribonuclease activities. Polysome-associated RNA contained 
      putative PHYA mRNA degradation products and was a mixture of polyadenylated and 
      deadenylated PHYA messages, suggesting that the two distinct degradation pathways 
      are polysome associated.
FAU - Higgs, D C
AU  - Higgs DC
AD  - Department of Botany, Iowa State University, Ames 50011-1020.
FAU - Colbert, J T
AU  - Colbert JT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Phytochrome A)
RN  - 0 (RNA, Messenger)
RN  - 11121-56-5 (Phytochrome)
RN  - 24937-83-5 (Poly A)
RN  - EC 3.1.- (Exoribonucleases)
RN  - EC 3.1.13.- (5'-exoribonuclease)
RN  - EC 3.1.26.4 (Ribonuclease H)
RN  - EC 3.2.1.31 (Glucuronidase)
SB  - IM
MH  - Base Sequence
MH  - Edible Grain/*metabolism
MH  - Exoribonucleases/metabolism
MH  - Glucuronidase/biosynthesis
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides
MH  - Phytochrome/*biosynthesis
MH  - Phytochrome A
MH  - Poly A/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Ribonuclease H/metabolism
PMC - PMC160496
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
PMCR- 1995/07/01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
PHST- 1995/07/01 00:00 [pmc-release]
AID - 6/7/1007 [pii]
AID - 10.1105/tpc.6.7.1007 [doi]
PST - ppublish
SO  - Plant Cell. 1994 Jul;6(7):1007-19. doi: 10.1105/tpc.6.7.1007.