PMID- 7915180
OWN - NLM
STAT- MEDLINE
DCOM- 19940928
LR  - 20190920
IS  - 0169-5002 (Print)
IS  - 0169-5002 (Linking)
VI  - 10
IP  - 1-2
DP  - 1993 Oct
TI  - Immunohistochemical study of P-glycoprotein distribution in lung cancer.
PG  - 1-12
AB  - Indirect immunoperoxidase was used to determine the reactivity of C219 
      (P-glycoCHEK C219, Centocor Diagnostics, Malvern, PA), a monoclonal antibody 
      (Mab) with high affinity for an internal epitope of the P-glycoprotein encoded by 
      the multidrug resistance (MDR1) gene, in 40 surgically resected primary lung 
      tumours. C219 reactivity was qualitatively classified in seven small cell lung 
      cancers (SCLC), 29 non small cell lung cancers (NSCLC), and four carcinoid 
      tumours. Ploidy was analysed by means of static cytometry using a 
      computer-assisted image processor following Feulgen staining of cytologic prints 
      of 32/40 lung tumours. Indirect immunoperoxidase reactivities of Mabs S-L 11.14 
      and MOC-1 were also studied to characterize the expression of cluster 1 lung 
      cancer antigens and hence to determine among the NSCLC those which expressed the 
      neural cell adhesion molecule (NCAM). Eighteen (45%) lung tumours strongly 
      expressed P-glycoprotein as an immunostaining of many islets of malignant cells 
      or almost all malignant cells. In addition, 8/40 tumours (20%) showed a weak 
      reactivity (few immunostained cells) and 14/40 (35%) no reactivity. There was no 
      difference of reactivity when NSCLC were compared with SCLC. The expression of 
      P-glycoprotein in NSCLC did not vary significantly when the stage of disease was 
      considered. Among the 29 NSCLC, 10 (36%) expressed S-L 11.14 and MOC-1. The NCAM 
      positive NSCLC did not show any difference of P-glycoprotein expression in 
      comparison with NCAM negative ones. Finally, C219 immunoperoxidase reactivity did 
      not significantly differ according to the ploidy status. In conclusion, the 
      internal epitope of the P-glycoprotein encoded by the MDR1 gene is frequently 
      expressed by lung tumours of any histological type. This expression is not higher 
      in Stage III and IV lung cancers in comparison with Stage I and II ones, or in 
      NSCLC in comparison with SCLC either. Thus, the C219 related epitope seems to 
      have a weak implication in the lower chemosensitivity of both advanced stages and 
      NSCLC.
FAU - Pujol, J L
AU  - Pujol JL
AD  - Centre Hospitalier Universitaire, Hopital Arnaud de Villeneuve, Montpellier, 
      France.
FAU - Simony, J
AU  - Simony J
FAU - Gautier, V
AU  - Gautier V
FAU - Marty-Ane, C
AU  - Marty-Ane C
FAU - Pujol, H
AU  - Pujol H
FAU - Michel, F B
AU  - Michel FB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Lung Cancer
JT  - Lung cancer (Amsterdam, Netherlands)
JID - 8800805
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 1)
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 1
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Non-Small-Cell Lung/chemistry
MH  - Carcinoma, Small Cell/chemistry
MH  - Carrier Proteins/*analysis/genetics/immunology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Lung Neoplasms/*chemistry
MH  - Male
MH  - Membrane Glycoproteins/*analysis/genetics/immunology
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - RNA, Messenger/analysis
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 0169-5002(93)90304-G [pii]
AID - 10.1016/0169-5002(93)90304-g [doi]
PST - ppublish
SO  - Lung Cancer. 1993 Oct;10(1-2):1-12. doi: 10.1016/0169-5002(93)90304-g.