PMID- 7915394
OWN - NLM
STAT- MEDLINE
DCOM- 19940926
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 55
IP  - 11
DP  - 1994
TI  - Dissimilar efficacy of opioids to produce mu-mediated analgesia: role of Gx/z and 
      Gi2 transducer proteins.
PG  - PL205-12
AB  - Intracerebroventricular (i.c.v.) administration to mice of IgGs raised against 
      alpha subunits of Gi2 or Gx/z transducer proteins lessened the activation of low 
      Km GTPase induced by morphine, DAMGO and DADLE in P2 membranes from mouse 
      periaqueductal grey matter (PAG). In mice injected with anti Gi2 alpha, DADLE, 
      DPDPE and [D-Ala2] Deltorphin II, but not beta-endorphin-(1-31), antagonized the 
      analgesic activity of morphine. Conversely, following anti Gx/z alpha, morphine 
      antagonized the antinociceptive potency of DADLE. It is concluded that opioids 
      display diverse efficacy at mu-Gi2 and mu-Gx/z complexes to produce supraspinal 
      analgesia in mice.
FAU - Garzon, J
AU  - Garzon J
AD  - Instituto Cajal, C.S.I.C., Madrid, Spain.
FAU - Martinez-Pena, Y
AU  - Martinez-Pena Y
FAU - Sanchez-Blazquez, P
AU  - Sanchez-Blazquez P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Endorphins)
RN  - 0 (Enkephalins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Opioid, mu)
RN  - 100929-53-1 (Enkephalin, Ala(2)-MePhe(4)-Gly(5)-)
RN  - 36B82AMQ7N (Naloxone)
RN  - 63631-40-3 (Enkephalin, Leucine-2-Alanine)
RN  - 76I7G6D29C (Morphine)
RN  - 88373-73-3 (Enkephalin, D-Penicillamine (2,5)-)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Analgesia
MH  - Analgesics, Opioid/*pharmacology
MH  - Animals
MH  - Cell Membrane/enzymology
MH  - Cerebral Ventricles/drug effects/*physiology
MH  - Endorphins/pharmacology
MH  - Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
MH  - Enkephalin, D-Penicillamine (2,5)-
MH  - Enkephalin, Leucine-2-Alanine/pharmacology
MH  - Enkephalins/pharmacology
MH  - GTP Phosphohydrolases/*metabolism
MH  - GTP-Binding Proteins/immunology/*metabolism
MH  - Immunoglobulin G/administration & dosage/*pharmacology
MH  - Injections, Intraventricular
MH  - Kinetics
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Morphine/antagonists & inhibitors/pharmacology
MH  - Naloxone/pharmacology
MH  - Pain/physiopathology
MH  - Peptide Fragments/immunology
MH  - Receptors, Opioid, mu/immunology/*physiology
MH  - *Signal Transduction
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 0024-3205(94)90048-5 [pii]
AID - 10.1016/0024-3205(94)90048-5 [doi]
PST - ppublish
SO  - Life Sci. 1994;55(11):PL205-12. doi: 10.1016/0024-3205(94)90048-5.