PMID- 7916971
OWN - NLM
STAT- MEDLINE
DCOM- 19941117
LR  - 20191023
IS  - 0952-5041 (Print)
IS  - 0952-5041 (Linking)
VI  - 12
IP  - 3
DP  - 1994 Jun
TI  - Response element selectivity for heterodimerization of vitamin D receptors with 
      retinoic acid and retinoid X receptors.
PG  - 327-39
AB  - The transcription of vitamin D (VD) responsive genes is regulated by three 
      different nuclear signalling pathways mediated by homodimers of VD receptors 
      (VDRs), heterodimers of VDRs and retinoid X receptors (RXRs) and heterodimers of 
      VDRs with retinoic acid receptors (RARs), Here, the in vitro DNA-binding affinity 
      of all three receptor complexes was shown to be enhanced by the presence of VD. 
      However, the specificity of the three pathways was dictated by the differential 
      affinities of the receptor complexes for VD response elements. Potential response 
      elements were distinguished by the sequence, the separation and the relative 
      orientation of the hexameric core binding motifs. It was found that both VDR-RAR 
      and VDR-RXR heterodimers act functionally on all three response element 
      configurations: direct repeats, palindromes and inverted palindromes. With direct 
      repeats, neither heterodimer type showed a preference for any of the three 
      principal core motifs, (A/G)GGTGA, (A/G)GGTCA and (A/G)GTTCA. However, while they 
      did exhibit preferences for core motifs in palindromes, the spacing requirements 
      were identical for both complexes. Inverted palindromes, however, formed the most 
      specific response elements. A simple model explains a steric link between the 
      optimal spacing of direct repeats and that of inverted palindromes. Taken 
      together, the experimental data and the model provide further criteria for the 
      screening of VD responsive genes.
FAU - Schrader, M
AU  - Schrader M
AD  - Clinique de Dermatologie, Hopital Cantonal Universitaire, Geneve, Switzerland.
FAU - Muller, K M
AU  - Muller KM
FAU - Becker-Andre, M
AU  - Becker-Andre M
FAU - Carlberg, C
AU  - Carlberg C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (DNA, Recombinant)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - 1406-16-2 (Vitamin D)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding, Competitive
MH  - Cell Line
MH  - DNA, Recombinant/genetics/metabolism
MH  - Drosophila
MH  - *Gene Expression Regulation/drug effects
MH  - Genes, Synthetic
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - *Protein Conformation
MH  - Receptors, Calcitriol/chemistry/*physiology
MH  - Receptors, Cytoplasmic and Nuclear/chemistry/*physiology
MH  - Receptors, Retinoic Acid/chemistry/*physiology
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - *Regulatory Sequences, Nucleic Acid
MH  - Repetitive Sequences, Nucleic Acid
MH  - Retinoid X Receptors
MH  - *Transcription Factors
MH  - Vitamin D/*pharmacology
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1677/jme.0.0120327 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 1994 Jun;12(3):327-39. doi: 10.1677/jme.0.0120327.