PMID- 7917274
OWN - NLM
STAT- MEDLINE
DCOM- 19941118
LR  - 20061115
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 38
IP  - 3
DP  - 1994 Jul
TI  - Analysis of segregation in a human male reciprocal translocation carrier, t(1;11) 
      (p36.3;q13.1), by two-colour fluorescence in situ hybridization.
PG  - 247-50
AB  - Using centromeric probes specific for chromosomes 1 and 11, 13,071 sperm nuclei 
      from a male reciprocal translocation heterozygote, 46,XY,t(1;11) (p36.3;q13.1), 
      were analyzed by fluorescence in situ hybridization (FISH). Decondensed sperm 
      nuclei were simultaneously hybridized with DNA probes for chromosome 1 (pUC177) 
      and chromosome 11 (D11Z1). Results were as follows: 1/11 (82.45%), 1/1/- (3.45%), 
      -/11/11 (4.85%), 1/1/11 (1.20%), 1/11/11 (1.14%), 1/- (4.33%), -/11 (2.50%), 
      1/1/11/11 (0.06%), 1/1/1/- (0.02%). Because both the normal chromosome and its 
      translocated derivative carry the same centromeric sequences, FISH cannot 
      differentiate between sperm resulting from alternate segregation and those 
      produced by adjacent I segregation. Using the same donor, comparable segregation 
      patterns were obtained from sperm chromosome karyotypes (Spriggs et al., 1992: 
      Hum Genet 88:447-452) and from MII spermatocytes (Goldman and Hulten, 1993: 
      Cytogenet Cell Genet 63:16-23), demonstrating that selection is not a factor in 
      the human sperm/hamster oocyte fusion technique or during meiosis. Although FISH 
      does not provide the detailed information afforded by sperm karyotyping, it is a 
      valuable technique for studying segregation patterns in translocation 
      heterozygotes.
FAU - Spriggs, E L
AU  - Spriggs EL
AD  - Department of Pediatrics, Faculty of Medicine, University of Calgary, Alberta, 
      Canada.
FAU - Martin, R H
AU  - Martin RH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
SB  - IM
MH  - Cell Nucleus/ultrastructure
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 11
MH  - Heterozygote
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Meiosis
MH  - Spermatozoa/ultrastructure
MH  - *Translocation, Genetic
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1002/mrd.1080380303 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 1994 Jul;38(3):247-50. doi: 10.1002/mrd.1080380303.