PMID- 7918534
OWN - NLM
STAT- MEDLINE
DCOM- 19941025
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1187
IP  - 3
DP  - 1994 Sep 27
TI  - The mitochondrial ATP/ADP carrier: interaction with detergents and purification 
      by a novel procedure.
PG  - 360-7
AB  - The interaction of several classes of detergents with mitochondrial ATP/ADP 
      carrier (AAC) was studied. The detergents that were best suited for 
      solubilization of active AAC differed in several physico-chemical properties, but 
      contained relatively rigid or planar hydrophobic (sub)moieties. Based on specific 
      binding of AAC to Blue Sepharose, a novel method for the purification of the AAC 
      was developed. The new method gave AAC samples which were devoid of non-essential 
      lipids and allowed to purify AAC isoenzymes from several species and tissues to a 
      significantly higher degree of purity than that achieved up to now. Western blot 
      analysis of purified AACs with an antiserum against chicken heart AAC confirmed 
      that immunological variability is more important between tissues than between 
      species. In contrast to liver and kidney AACs, brain AAC displayed similar 
      antigenic properties to heart AAC.
FAU - Rojo, M
AU  - Rojo M
AD  - Institute for Cell Biology, Swiss Federal Institute of Technology, Zurich.
FAU - Wallimann, T
AU  - Wallimann T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Detergents)
RN  - 9068-80-8 (Mitochondrial ADP, ATP Translocases)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Fractionation
MH  - Chickens
MH  - Detergents
MH  - Mitochondria/*enzymology
MH  - Mitochondria, Heart/enzymology
MH  - Mitochondria, Liver/enzymology
MH  - Mitochondria, Muscle/enzymology
MH  - Mitochondrial ADP, ATP Translocases/*isolation & purification
MH  - Rats
EDAT- 1994/09/27 00:00
MHDA- 1994/09/27 00:01
CRDT- 1994/09/27 00:00
PHST- 1994/09/27 00:00 [pubmed]
PHST- 1994/09/27 00:01 [medline]
PHST- 1994/09/27 00:00 [entrez]
AID - 0005-2728(94)90010-8 [pii]
AID - 10.1016/0005-2728(94)90010-8 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1994 Sep 27;1187(3):360-7. doi: 
      10.1016/0005-2728(94)90010-8.