PMID- 7918672
OWN - NLM
STAT- MEDLINE
DCOM- 19941101
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1223
IP  - 3
DP  - 1994 Sep 29
TI  - Annexin VI isoforms are differentially expressed in mammalian tissues.
PG  - 368-74
AB  - Purified annexin VI migrates as a closely spaced doublet when separated by 
      SDS-PAGE. Immunolocalization of annexin VI in heart demonstrates staining at 
      different defined subcellular compartments. Moss et al. identified two cDNAs, one 
      having an insert of 18 bases encoding VAAEIL at the beginning of repeat domain 
      seven. We have identified the splicing site of the murine annexin VI gene. It 
      contains a single small exon of 18 bases. PCR amplification of reverse 
      transcribed (RT) mRNA demonstrates that, in all tissues tested, the mRNA isoform 
      containing the insert is predominant. Site-directed antibody was produced and 
      affinity purified against peptides reflecting the insert and deletion sequences. 
      The steady-state isoform ratio of the annexin VI protein is consistent with the 
      RT-PCR data. Chromatographic experiments demonstrate that the annexin VI protein 
      isoforms have biochemical differences. These differences may target the 
      individual isoforms to unique cellular compartments or alter functional 
      properties.
FAU - Kaetzel, M A
AU  - Kaetzel MA
AD  - Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 
      OH 45267-576.
FAU - Pula, G
AU  - Pula G
FAU - Campos, B
AU  - Campos B
FAU - Uhrin, P
AU  - Uhrin P
FAU - Horseman, N
AU  - Horseman N
FAU - Dedman, J R
AU  - Dedman JR
LA  - eng
GR  - DK 41740/DK/NIDDK NIH HHS/United States
GR  - DK 46433/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Annexin A6)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Annexin A6/chemistry/genetics/*metabolism
MH  - Base Sequence
MH  - DNA, Complementary/analysis
MH  - Fluorescent Antibody Technique
MH  - Gene Expression
MH  - Mice
MH  - Molecular Sequence Data
MH  - Myocardium/*metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
EDAT- 1994/09/29 00:00
MHDA- 1994/09/29 00:01
CRDT- 1994/09/29 00:00
PHST- 1994/09/29 00:00 [pubmed]
PHST- 1994/09/29 00:01 [medline]
PHST- 1994/09/29 00:00 [entrez]
AID - 0167-4889(94)90097-3 [pii]
AID - 10.1016/0167-4889(94)90097-3 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1994 Sep 29;1223(3):368-74. doi: 
      10.1016/0167-4889(94)90097-3.