PMID- 7919140
OWN - NLM
STAT- MEDLINE
DCOM- 19941028
LR  - 20210518
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 4
IP  - 11
DP  - 1994 May
TI  - The effect of uremia on tumor necrosis factor-alpha release after an in vitro 
      whole-blood endotoxin challenge.
PG  - 1890-5
AB  - Uremia has been associated with immunologic aberrations, including anergy, 
      increased susceptibility to infections, and reduced phagocytic activity of 
      polymorphonuclear leukocytes. In this study, cytokine release in uremic and 
      nonuremic blood after in vitro endotoxin stimulation was studied. Blood from 
      nonuremic controls, chronic renal failure patients not on dialysis, and chronic 
      hemodialysis patients predialysis and postdialysis was spiked with 10 ng/mL of 
      Escherichia coli endotoxin and incubated for 2 and 26 h. Plasma tumor necrosis 
      factor-alpha (TNF alpha) concentrations were determined by ELISA after each 
      incubation period. To further study which uremic blood component may be 
      responsible for enhanced release of TNF alpha, plasma and cellular components of 
      chronic renal failure patients and controls were switched and then given an in 
      vitro endotoxin stimulation (1 ng/mL). It was found that (1) TNF alpha release is 
      enhanced by uremia and is exacerbated with progressive declines in renal 
      function, (2) enhanced TNF alpha release is related to a blood cellular 
      phenomenon induced by uremia, and (3) enhanced TNF alpha release in hemodialysis 
      patients is associated with a prolonged stimulation and/or reduced plasma 
      elimination of TNF alpha.
FAU - Oettinger, C W
AU  - Oettinger CW
AD  - Emory University School of Medicine, Atlanta, GA.
FAU - Bland, L A
AU  - Bland LA
FAU - Oliver, J C
AU  - Oliver JC
FAU - Arduino, M J
AU  - Arduino MJ
FAU - McAllister, S K
AU  - McAllister SK
FAU - Favero, M S
AU  - Favero MS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (Endotoxins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Blood Cells/immunology
MH  - Endotoxins/*toxicity
MH  - Humans
MH  - In Vitro Techniques
MH  - Models, Biological
MH  - Plasma/immunology
MH  - Renal Dialysis
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - Uremia/blood/*immunology/therapy
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 10.1681/ASN.V4111890 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 1994 May;4(11):1890-5. doi: 10.1681/ASN.V4111890.