PMID- 7919389
OWN - NLM
STAT- MEDLINE
DCOM- 19941109
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 84
IP  - 8
DP  - 1994 Oct 15
TI  - Monocyte chemoattractant protein-1 gene is expressed in activated neutrophils and 
      retinoic acid-induced human myeloid cell lines.
PG  - 2776-83
AB  - We have used differential display polymerase chain reaction to identify genes 
      that are upregulated after retinoic acid (RA) treatment of human myeloblastic 
      HL-60 cells. Three of the cDNAs cloned hybridized to RA-inducible transcripts on 
      Northern blots, one of which was shown to encode sequences for monocyte 
      chemoattractant protein-1 (MCP-1), a recently described cytokine that is 
      chemotactic for monocytes but not for neutrophils. Nuclear run-on analysis 
      indicated that the upregulation of the MCP-1 gene occurs at the transcriptional 
      level in HL-60 cells. MCP-1 transcript levels also increased after RA treatment 
      of the NB4 acute promyelocytic cell line. MCP-1 transcripts were undetectable in 
      freshly isolated neutrophils by Northern analysis or reverse 
      transcription-polymerase chain reaction but were readily detectable in 
      neutrophils after incubation in media at 37 degrees C for 20 hours, suggesting 
      that an activation event can lead to MCP-1 expression in neutrophils. 
      Immunocytochemistry confirmed the presence of MCP-1 protein in activated 
      neutrophils. This is the first report that the MCP-1 gene is RA-responsive in 
      myeloid cell lines and is expressed in neutrophils. MCP-1 expression by activated 
      neutrophils may play an important role in attracting monocytes to the site of 
      tissue damage or infection.
FAU - Burn, T C
AU  - Burn TC
AD  - Hematology/Oncology Division, Beth Israel Hospital, Boston, MA 02215.
FAU - Petrovick, M S
AU  - Petrovick MS
FAU - Hohaus, S
AU  - Hohaus S
FAU - Rollins, B J
AU  - Rollins BJ
FAU - Tenen, D G
AU  - Tenen DG
LA  - eng
GR  - CA09161/CA/NCI NIH HHS/United States
GR  - CA34183/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*genetics
MH  - Gene Expression/*drug effects
MH  - Granulocytes/drug effects/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Leukemia, Promyelocytic, Acute
MH  - Molecular Sequence Data
MH  - Neutrophil Activation
MH  - Neutrophils/*metabolism
MH  - Polymerase Chain Reaction
MH  - Tretinoin/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1994/10/15 00:00
MHDA- 1994/10/15 00:01
CRDT- 1994/10/15 00:00
PHST- 1994/10/15 00:00 [pubmed]
PHST- 1994/10/15 00:01 [medline]
PHST- 1994/10/15 00:00 [entrez]
AID - S0006-4971(20)71399-6 [pii]
PST - ppublish
SO  - Blood. 1994 Oct 15;84(8):2776-83.