PMID- 7921415
OWN - NLM
STAT- MEDLINE
DCOM- 19941110
LR  - 20190920
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 425
IP  - 1
DP  - 1994
TI  - Over-expression of glucose transporter isoform GLUT1 and hexokinase I in rat 
      renal oncocytic tubules and oncocytomas.
PG  - 63-8
AB  - Renal oncocytomas, which have previously been shown to originate from the 
      collecting duct system, were induced in male Sprague-Dawley rats by oral 
      administration of N-nitrosomorpholine (NNM) for 7 weeks. The expression of 
      glucose transporter isoforms GLUT1 and GLUT2, and of several enzymes involved in 
      glucose metabolism [hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase 
      (LDH), malate dehydrogenase (MDH)] were studied by cytochemical approaches in 
      serial cryostat sections of the kidney 12, 23 and 34 weeks after withdrawal of 
      NNM. Oncocytic tubules connected with collecting ducts were first observed 23 
      weeks, and oncocytomas 34 weeks after withdrawal. The cytochemical pattern of 
      oncocytic tubules and oncocytomas was similar, but differed markedly from that of 
      normal collecting ducts in nearly all variables studied; expression of GLUT1 and 
      hexokinase I proteins were strongly increased; activities of HK, PK and MDH were 
      elevated, while LDH activity was reduced. These results suggest that oncocytic 
      transformation is associated with fundamental changes in energy metabolism which 
      differ from those in cell lineages leading to other types of renal cell tumours, 
      such as clear/acidophilic and basophilic cell tumours. The characteristic 
      over-expression of GLUT1 may be used as a diagnostic criterion for the 
      discrimination between oncocytes and acidophilic (granular) cells in 
      clear/acidophilic renal cell tumours which show a reduced expression of this 
      glucose transporter protein.
FAU - Ahn, Y S
AU  - Ahn YS
AD  - Abteilung fur Cytopathologie, Deutsches Krebsforschungszentrum, Heidelberg, 
      Germany.
FAU - Rempel, A
AU  - Rempel A
FAU - Zerban, H
AU  - Zerban H
FAU - Bannasch, P
AU  - Bannasch P
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (Isoenzymes)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Slc2a1 protein, rat)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Glucose Transporter Type 1
MH  - Hexokinase/*metabolism
MH  - Histocytochemistry
MH  - Isoenzymes/*metabolism
MH  - Kidney Neoplasms/*metabolism/pathology
MH  - Kidney Tubules/*metabolism/pathology
MH  - Male
MH  - Monosaccharide Transport Proteins/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reference Values
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1007/BF00193950 [doi]
PST - ppublish
SO  - Virchows Arch. 1994;425(1):63-8. doi: 10.1007/BF00193950.