PMID- 7921446
OWN - NLM
STAT- MEDLINE
DCOM- 19941110
LR  - 20131121
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 150
IP  - 4
DP  - 1994 Oct
TI  - Inhibition of lung immunity after intratracheal instillation of benzo(a)pyrene.
PG  - 1123-9
AB  - Benzo(a)pyrene (B(a)P) has been shown to suppress systemic immunity in 
      experimental animals, which may contribute to the growth of the chemical-induced 
      tumors. However, its effects on lung immunity after inhalation, a common route 
      for human exposure in urban areas, has not been determined. These studies examine 
      intratracheal B(a)P instillation on lung natural killer (NK) cell activity, 
      alveolar macrophage (AM) functions, and susceptibility to tumor cell challenge in 
      Fischer 344 (F-344) rats. Adult female F-344 rats were given a single 
      intratracheal instillation of 0, 10, 20, or 40 mg B(a)P/kg body weight as a 
      suspension, and lung NK cell activity and AM functions were examined 7, 21, or 
      100 d later. Although exposure to B(a)P did not alter cell recovery after lavage, 
      histologic changes were observed as evidenced by granulomatous inflammation and 
      squamous metaplasia. There was a slight but significant suppression of H2O2 and 
      nitric oxide (NO) release from alveolar macrophages of treated animals as well as 
      NK cell activity from the lung digest. A marked suppression of tumor necrosis 
      factor-alpha (TNF alpha) and interleukin (IL-1) secretion in LPS- and/or 
      cytokine-activated alveolar macrophages occurred. The suppressive effects were 
      generally more severe on Day 7 after exposure than on Days 21 or 100, although 
      IL-1 remained depressed through Day 100 after exposure. B(a)P exposure allowed 
      for the increased growth of MADB106 metastatic tumor cells in the lung. These 
      tumor cells were shown to be highly sensitive to lysis by immune-mediators, 
      including TNF alpha.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Kong, L Y
AU  - Kong LY
AD  - Environmental Immunology and Neurobiology Section, National Institute of 
      Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
FAU - Luster, M I
AU  - Luster MI
FAU - Dixon, D
AU  - Dixon D
FAU - O'Grady, J
AU  - O'Grady J
FAU - Rosenthal, G J
AU  - Rosenthal GJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 3417WMA06D (Benzo(a)pyrene)
SB  - IM
MH  - Adenocarcinoma/immunology/secondary
MH  - Animals
MH  - Benzo(a)pyrene/*administration & dosage
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Depression, Chemical
MH  - Female
MH  - Immunity, Cellular/drug effects
MH  - Instillation, Drug
MH  - Killer Cells, Natural/drug effects/immunology
MH  - Lung/*drug effects/*immunology
MH  - Lung Neoplasms/immunology/secondary
MH  - Macrophages, Alveolar/drug effects/immunology
MH  - Neoplasm Transplantation
MH  - Phagocytosis/drug effects
MH  - Random Allocation
MH  - Rats
MH  - Rats, Inbred F344
MH  - Time Factors
MH  - Trachea
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1164/ajrccm.150.4.7921446 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 1994 Oct;150(4):1123-9. doi: 
      10.1164/ajrccm.150.4.7921446.