PMID- 7922786
OWN - NLM
STAT- MEDLINE
DCOM- 19941117
LR  - 20190830
IS  - 0171-967X (Print)
IS  - 0171-967X (Linking)
VI  - 55
IP  - 1
DP  - 1994 Jul
TI  - Modulation of vitamin D increased H2O2 production and MAC-2 expression in the 
      bone marrow-derived macrophages by estrogen.
PG  - 29-32
AB  - The calcium-regulating hormone 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) is 
      recognized as an immunomodulator. Members of the macrophage-monocyte lineage are 
      targets for 1,25(OH)2D3 action. The hormone enhances the ability of bone 
      marrow-derived macrophages (BMDMs) to produce H2O2, increases the expression of 
      the macrophage specific surface antigen MAC-2, increases the release of tumor 
      necrosis factor-alpha (TNF-alpha), and inhibits BMDM proliferation. In the 
      present study we examine the possibility that estrogen modulates 1,25(OH)2D3 
      effects on BMDMs. The active form, 17 beta-estradiol, failed to affect any of the 
      BMDM functions by itself. On the other hand, 17 beta-estradiol increased the 
      effects of 1,25(OH)2D3 production by BMDMs and on MAC-2 expression on these 
      cells. The inactive estrogen analog 17 alpha-estradiol was unable to elicit these 
      effects. Moreover, 17 beta-estradiol was unable to elicit these effects. 
      Moreover, 17 beta-estradiol did not affect the lipopolysaccharide (LPS)-induced 
      increase in H2O2 production by BMDMs. Modulation of BMDM proliferation and 
      TNF-alpha release from these cells by 1,25(OH)2D3 were not affected by the 
      estrogen. The experiments were performed with BMDMs harvested from vitamin 
      D-depleted and repleted mice, and always under similar conditions, the various 
      functions were more pronounced in the cells derived from the repleted mice. Our 
      data are consistent with the hypothesis that 17 beta-estradiol modulates the 
      interactions of 1,25(OH)2D3 with BMDMs and consequently is able to affect 
      biological responses to 1,25(OH)2D3 in these cells. We propose that this cell 
      system is a convenient, nontransformed model for studying cellular activities of 
      1,25(OH)2D3.
FAU - Abu-Amer, Y
AU  - Abu-Amer Y
AD  - Hubert Humphrey Center for Experimental Medicine and Cancer Research, Hebrew 
      University-Hadassah Medical School, Jerusalem, Israel.
FAU - Bar-Shavit, Z
AU  - Bar-Shavit Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Calcif Tissue Int
JT  - Calcified tissue international
JID - 7905481
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Galectin 3)
RN  - 0 (Lipopolysaccharides)
RN  - 4TI98Z838E (Estradiol)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/*biosynthesis/genetics
MH  - Bone Marrow/drug effects
MH  - Bone Marrow Cells
MH  - Calcitriol/*metabolism/pharmacology
MH  - Cell Division/drug effects
MH  - Culture Techniques
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Estradiol/*pharmacology
MH  - Galectin 3
MH  - Gene Expression/drug effects/genetics
MH  - Hydrogen Peroxide/*metabolism
MH  - Lipopolysaccharides/toxicity
MH  - Macrophages/drug effects/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Vitamin D Deficiency/metabolism
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1007/BF00310165 [doi]
PST - ppublish
SO  - Calcif Tissue Int. 1994 Jul;55(1):29-32. doi: 10.1007/BF00310165.