PMID- 7923076
OWN - NLM
STAT- MEDLINE
DCOM- 19941116
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 77
IP  - 1
DP  - 1994 Oct
TI  - Cytogenetic and fluorescence in situ hybridization studies on sporadic and 
      hereditary tumors associated with von Hippel-Lindau syndrome (VHL).
PG  - 1-13
AB  - We performed cytogenetic and fluorescence in situ hybridization (FISH) studies on 
      29 sporadic or familial tumors associated with von Hippel-Lindau [correction of 
      Landau] disease. Four of five renal cell carcinomas with detectable alterations 
      showed clones with chromosome 3 alterations. These changes led to loss of genetic 
      material visible with cytogenetic resolution: either an unbalanced translocation 
      involving 3p or loss of a whole homolog 3, resulting in monosomy of 3p. We have 
      previously mapped the VHL gene to chromosomal region 3p25-p26. We applied FISH 
      using the single copy probes cA233 and cA479, sequences close to the VHL gene, in 
      a search for submicroscopic deletions of 3p. Use of FISH with differentially 
      labeled probes indicated cA479 to be distal to cA233, but both were located 
      within bands 3p25-26. FISH with single copy probes for interphase cytogenetics 
      detected four subclones with deletions in the VHL region in 8/22 tumors, 
      including four tumors which appeared cytogenetically normal. FISH proved to be a 
      powerful tool in tumor genetic studies, especially helpful in detecting tumor 
      subclones in benign and slowly growing tumors.
FAU - Decker, H J
AU  - Decker HJ
AD  - Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Boston.
FAU - Klauck, S M
AU  - Klauck SM
FAU - Lawrence, J B
AU  - Lawrence JB
FAU - McNeil, J
AU  - McNeil J
FAU - Smith, D
AU  - Smith D
FAU - Gemmill, R M
AU  - Gemmill RM
FAU - Sandberg, A A
AU  - Sandberg AA
FAU - Neumann, H H
AU  - Neumann HH
FAU - Simon, B
AU  - Simon B
FAU - Green, J
AU  - Green J
AU  - et al.
LA  - eng
GR  - CA 20051/CA/NCI NIH HHS/United States
GR  - CA-41183/CA/NCI NIH HHS/United States
GR  - R0I-CA 49455/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adrenal Gland Neoplasms/genetics
MH  - Angiomatosis/genetics
MH  - Carcinoma, Renal Cell/genetics
MH  - Cerebellar Neoplasms/genetics
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - *Chromosomes, Human, Pair 3
MH  - Hemangioblastoma/genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Kidney Neoplasms/genetics
MH  - Neoplastic Syndromes, Hereditary/*genetics
MH  - Pheochromocytoma/genetics
MH  - Polymorphism, Restriction Fragment Length
MH  - Retinal Diseases/genetics
MH  - Tumor Cells, Cultured
MH  - von Hippel-Lindau Disease/*genetics
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 0165-4608(94)90141-4 [pii]
AID - 10.1016/0165-4608(94)90141-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1994 Oct;77(1):1-13. doi: 10.1016/0165-4608(94)90141-4.