PMID- 7924686
OWN - NLM
STAT- MEDLINE
DCOM- 19941028
LR  - 20131121
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 16
IP  - 2
DP  - 1994 Jun 1
TI  - Improved technique for analysis of formalin-fixed, paraffin-embedded tumors by 
      fluorescence in situ hybridization.
PG  - 93-9
AB  - Fluorescence in situ hybridization (FISH) and specific DNA probes for 
      peri-centromeric repeat regions and unique sequence loci have made it possible to 
      study chromosomal aberrations from interphase tumor nuclei. Large-scale 
      retrospective studies on the prognostic value of interphase cytogenetics would 
      become feasible if these techniques were readily applicable to nuclei from 
      archival formalin-fixed tumor tissues. We describe here an improved technique for 
      interphase FISH analysis of tumors that have been extensively fixed in formalin. 
      The protocol aims at improving probe penetration and hybridization efficiency by 
      inducing chromatin decondensation and swelling of the nuclei with a heat 
      treatment in a 90 degrees C glycerol solution prior to hybridization. Using this 
      cell pretreatment, FISH results on the detection of chromosome copy number 
      aberrations and amplification of the c-erbB-2 oncogene from formalin-fixed, 
      paraffin-embedded tissues were highly concordant with those from fresh tissues. 
      In contrast to previously described methods, separate adjustments of denaturation 
      or proteinase K digestion are not required for each sample. This method 
      facilitates retrospective analyses of large series of tumors and is also useful 
      for applying FISH to routine diagnostic purposes using formalin-fixed material.
FAU - Hyytinen, E
AU  - Hyytinen E
AD  - Department of Clinical Chemistry, Tampere University Hospital, Finland.
FAU - Visakorpi, T
AU  - Visakorpi T
FAU - Kallioniemi, A
AU  - Kallioniemi A
FAU - Kallioniemi, O P
AU  - Kallioniemi OP
FAU - Isola, J J
AU  - Isola JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Chromatin)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 1HG84L3525 (Formaldehyde)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
GS  - c-erbB-2
MH  - Breast Neoplasms/genetics/pathology
MH  - Centromere/ultrastructure
MH  - Chromatin/drug effects/ultrastructure
MH  - Chromosomes, Human/drug effects/ultrastructure
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis
MH  - *Formaldehyde
MH  - Gene Amplification
MH  - Glycerol/pharmacology
MH  - Hot Temperature
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Microscopy, Fluorescence/instrumentation
MH  - Neoplasms/genetics/*pathology
MH  - Oncogenes
MH  - *Paraffin Embedding
MH  - Repetitive Sequences, Nucleic Acid
MH  - Retrospective Studies
MH  - *Tissue Fixation
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1002/cyto.990160202 [doi]
PST - ppublish
SO  - Cytometry. 1994 Jun 1;16(2):93-9. doi: 10.1002/cyto.990160202.