PMID- 7925278
OWN - NLM
STAT- MEDLINE
DCOM- 19941031
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 13
IP  - 18
DP  - 1994 Sep 15
TI  - The proteolysis-dependent metaphase to anaphase transition: 
      calcium/calmodulin-dependent protein kinase II mediates onset of anaphase in 
      extracts prepared from unfertilized Xenopus eggs.
PG  - 4343-52
AB  - It has been shown, using spindles assembled in vitro in extracts containing CSF 
      (the cytostatic factor responsible for arresting unfertilized vertebrate eggs at 
      metaphase), that onset of anaphase requires Ca(2+)-dependent activation of the 
      ubiquitin-dependent proteolytic pathway that destroys both mitotic cyclins and an 
      unknown protein responsible for metaphase arrest (Holloway et al., 1993, Cell, 
      73, 1382-1402). We showed recently that Ca2+/calmodulin-dependent protein kinase 
      II (CaM KII) activates the ubiquitin-dependent cyclin degradation pathway in CSF 
      extracts (Lorca et al., 1993, Nature, 366, 270-273), but did not investigate its 
      possible effect on sister chromatid segregation. In this work we identify CaM KII 
      as the only target of Ca2+ in inducing anaphase in CSF extracts, and further show 
      that transition to anaphase does not require the direct phosphorylation of 
      metaphase spindle components by CaM KII. A possible interpretation of the above 
      results could have been that the ubiquitin-dependent degradation pathway is 
      required for onset of anaphase only when spindles are clamped at metaphase due to 
      CSF activity, and not in the regular cell cycle that occurs in the absence of CSF 
      activity. We ruled out this possibility by showing that competitive inhibition of 
      the ubiquitin-dependent degradation pathway still prevents the onset of anaphase 
      in cycling extracts that lack CSF and do not require Ca2+ for sister chromatid 
      separation.
FAU - Morin, N
AU  - Morin N
AD  - CNRS UPR 9008/INSERM U 249, Montpellier, France.
FAU - Abrieu, A
AU  - Abrieu A
FAU - Lorca, T
AU  - Lorca T
FAU - Martin, F
AU  - Martin F
FAU - Doree, M
AU  - Doree M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Cyclins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Ubiquitins)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-mos)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Anaphase/*physiology
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Cell-Free System
MH  - Chromatids/physiology
MH  - Cyclins/genetics/metabolism
MH  - DNA Replication
MH  - Hydrolysis
MH  - Metaphase/*physiology
MH  - Motion
MH  - Ovum/*physiology
MH  - Peptide Fragments/pharmacology
MH  - Phosphorylation
MH  - Proteins/metabolism
MH  - Proto-Oncogene Proteins c-mos/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Spindle Apparatus/physiology
MH  - Ubiquitins/metabolism
MH  - Xenopus
PMC - PMC395361
EDAT- 1994/09/15 00:00
MHDA- 2000/03/23 09:00
PMCR- 1995/09/15
CRDT- 1994/09/15 00:00
PHST- 1994/09/15 00:00 [pubmed]
PHST- 2000/03/23 09:00 [medline]
PHST- 1994/09/15 00:00 [entrez]
PHST- 1995/09/15 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1994.tb06754.x [doi]
PST - ppublish
SO  - EMBO J. 1994 Sep 15;13(18):4343-52. doi: 10.1002/j.1460-2075.1994.tb06754.x.