PMID- 7925596
OWN - NLM
STAT- MEDLINE
DCOM- 19941021
LR  - 20191210
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 258
IP  - 1-2
DP  - 1994 Jun 2
TI  - Mastoparan reduces the supraspinal analgesia mediated by mu/delta-opioid 
      receptors in mice.
PG  - 159-62
AB  - Intracerebroventricular (i.c.v.) administration of the venom peptide, mastoparan, 
      to mice decreased to a limited extent opioid-induced supraspinal analgesia in a 
      non-competitive fashion. The mu-opioid receptor agonists, 
      [D-Ala2,N-MePhe4,Gly-ol5]-enkephalin (DAMGO) and morphine, the mu/delta-opioid 
      receptor ligands, human beta-endorphin-(1-31) and [D-Ala2,D-Leu5]-enkephalin 
      (DADLE), and the selective ligands of delta-opioid receptors, 
      [D-Pen2,5]enkephalin (DPDPE) and [D-Ala2]deltorphin II, showed an impaired 
      analgesic effect in mice given mastoparan. Mastoparan diminished the analgesic 
      activity of DPDPE and [D-Ala2]deltorphin II to the same extent as observed after 
      giving the delta-opioid receptor-selective antagonist, ICI 174864. The mu-opioid 
      receptor-mediated analgesia that remained after mastoparan was abolished in the 
      presence of the opioid antagonist, naloxone. Mastoparan after binding to Gi 
      alpha/Go alpha subunits could block opioid antinociception. The existence of a 
      class of G protein functionally coupled to mu-opioid receptors, but resistant to 
      the effect of mastoparan is suggested.
FAU - Sanchez-Blazquez, P
AU  - Sanchez-Blazquez P
AD  - Instituto Cajal, CSIC, Madrid, Spain.
FAU - Garzon, J
AU  - Garzon J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Analgesics)
RN  - 0 (Enkephalins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Opioid)
RN  - 0 (Wasp Venoms)
RN  - 100929-53-1 (Enkephalin, Ala(2)-MePhe(4)-Gly(5)-)
RN  - 63631-40-3 (Enkephalin, Leucine-2-Alanine)
RN  - 72093-21-1 (mastoparan)
RN  - 88373-73-3 (Enkephalin, D-Penicillamine (2,5)-)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Analgesics/*antagonists & inhibitors/pharmacology
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
MH  - Enkephalin, D-Penicillamine (2,5)-
MH  - Enkephalin, Leucine-2-Alanine/pharmacology
MH  - Enkephalins/pharmacology
MH  - GTP-Binding Proteins/metabolism
MH  - Injections, Intraventricular
MH  - Intercellular Signaling Peptides and Proteins
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptides
MH  - Receptors, Opioid/*drug effects/metabolism
MH  - Wasp Venoms/administration & dosage/*pharmacology
EDAT- 1994/06/02 00:00
MHDA- 1994/06/02 00:01
CRDT- 1994/06/02 00:00
PHST- 1994/06/02 00:00 [pubmed]
PHST- 1994/06/02 00:01 [medline]
PHST- 1994/06/02 00:00 [entrez]
AID - 0014-2999(94)90070-1 [pii]
AID - 10.1016/0014-2999(94)90070-1 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1994 Jun 2;258(1-2):159-62. doi: 10.1016/0014-2999(94)90070-1.