PMID- 7926306
OWN - NLM
STAT- MEDLINE
DCOM- 19941108
LR  - 20190515
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 43
IP  - 11
DP  - 1994 Nov
TI  - HLA-DQ-restricted, islet-specific T-cell clones of a type I diabetic patient. 
      T-cell receptor sequence similarities to insulitis-inducing T-cells of nonobese 
      diabetic mice.
PG  - 1318-25
AB  - We established a T-cell line and 20 CD4+ T-cell clones from the peripheral blood 
      lymphocytes of a type I diabetic patient using a membrane preparation of a rat 
      insulinoma cell line (beta membrane antigen [BMA]) as a source of antigen. The 
      T-cell line and three selected clones proliferated specifically to stimulation 
      with BMA and to membranes prepared from human islets, rat pancreas, and NOD 
      pancreas, but not to control antigens. Proliferation-inhibition studies using 
      human leukocyte antigen (HLA)-specific monoclonal antibodies revealed 
      HLA-DQw1-restricted recognition of BMA. An analysis of the T-cell receptor (TCR) 
      repertoire of the T-cell line after 8 and 40 days of culture showed a prominent 
      usage of the V alpha 1 and V alpha 12 gene families. Although sequencing of the 
      TCR V alpha and V beta chains of the three clones demonstrated that each used 
      different V alpha and V beta gene segments, structural similarities were found in 
      complementary-determining region 3 (CDR3), the region that is postulated to 
      interact with the peptide component of the TCR ligand. When we compared these TCR 
      sequences with published sequences of disease-inducing T-cells of NOD mice, 
      highly related TCR V beta families were detected. Furthermore, stretches of 
      identical amino acids within the CDR3 region were found between two pairs of 
      human and mouse T-cells. If one considers the species differences in TCR genes 
      and sequence differences in the restriction elements for these cells (HLA-DQ vs. 
      H-2 I-A nod), these sequence similarities are striking and may be useful for 
      pinpointing T-cells of primary importance in the development of disease.
FAU - Durinovic-Bello, I
AU  - Durinovic-Bello I
AD  - Institute of Diabetes Research, City Hospital Munich-Schwabing, Munich, Germany.
FAU - Steinle, A
AU  - Steinle A
FAU - Ziegler, A G
AU  - Ziegler AG
FAU - Schendel, D J
AU  - Schendel DJ
LA  - eng
SI  - GENBANK/S74026
SI  - GENBANK/S74027
SI  - GENBANK/S74028
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Clone Cells
MH  - Diabetes Mellitus, Type 1/*genetics/*immunology
MH  - Female
MH  - HLA-DQ Antigens/*immunology
MH  - Humans
MH  - Islets of Langerhans/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Molecular Sequence Data
MH  - Pedigree
MH  - Receptors, Antigen, T-Cell/*genetics
MH  - Sequence Homology, Amino Acid
MH  - T-Lymphocytes/*immunology
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.2337/diab.43.11.1318 [doi]
PST - ppublish
SO  - Diabetes. 1994 Nov;43(11):1318-25. doi: 10.2337/diab.43.11.1318.