PMID- 7927717
OWN - NLM
STAT- MEDLINE
DCOM- 19941104
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 62
IP  - 10
DP  - 1994 Oct
TI  - Identification of a locus involved in the utilization of iron by Haemophilus 
      influenzae.
PG  - 4515-25
AB  - Haemophilus influenzae has an absolute requirement for heme for aerobic growth. 
      This organism can satisfy this requirement by synthesizing heme from iron and 
      protoporphyrin IX (PPIX). H. influenzae type b (Hib) strain DL42 was found to be 
      unable to form single colonies when grown on a medium containing free iron and 
      PPIX in place of heme. In contrast, the nontypeable H. influenzae (NTHI) strain 
      TN106 grew readily on the same medium. A genomic library from NTHI strain TN106 
      was used to transform Hib strain DL42, and recombinants were selected on a medium 
      containing iron and PPIX in place of heme. A recombinant plasmid with an 11.5-kb 
      NTHI DNA insert was shown to confer on Hib strain DL42 the ability to grow on 
      iron and PPIX. Nucleotide sequence analysis revealed that this NTHI DNA insert 
      contained three genes, designated hitA, hitB, and hitC, which encoded products 
      similar to the SfuABC proteins of Serratia marcescens, which have been shown to 
      constitute a periplasmic binding protein-dependent iron transport system in this 
      enteric organism. The NTHI HitA protein also was 69% identical to the 
      ferric-binding protein of Neisseria gonorrhoeae. Inactivation of the cloned NTHI 
      hitC gene by insertion of an antibiotic resistance cartridge eliminated the 
      ability of the recombinant plasmid to complement the growth deficiency of Hib 
      DL42. Construction of an isogenic NTHI TN106 mutant lacking a functional hitC 
      gene revealed that this mutation prevented this strain from growing on a medium 
      containing iron and PPIX in place of heme. This NTHI hitC mutant was also unable 
      to utilize either iron bound to transferrin or iron chelates. These results 
      suggest that the products encoded by the hitABC genes are essential for the 
      utilization of iron by NTHI.
FAU - Sanders, J D
AU  - Sanders JD
AD  - Department of Microbiology, University of Texas Southwestern Medical Center, 
      Dallas 75235-9048.
FAU - Cope, L D
AU  - Cope LD
FAU - Hansen, E J
AU  - Hansen EJ
LA  - eng
GR  - AI17621/AI/NIAID NIH HHS/United States
GR  - AI23366/AI/NIAID NIH HHS/United States
GR  - NCI CA09082-19/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Bacterial Proteins)
RN  - 0 (Protoporphyrins)
RN  - C2K325S808 (protoporphyrin IX)
RN  - E1UOL152H7 (Iron)
SB  - IM
GS  - hitA
GS  - hitB
GS  - hitC
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/analysis/genetics
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - *Genes, Bacterial
MH  - Haemophilus influenzae/*genetics/growth & development/metabolism
MH  - Iron/*metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Protoporphyrins/pharmacology
PMC - PMC303138
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
PMCR- 1994/10/01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
PHST- 1994/10/01 00:00 [pmc-release]
AID - 10.1128/iai.62.10.4515-4525.1994 [doi]
PST - ppublish
SO  - Infect Immun. 1994 Oct;62(10):4515-25. doi: 10.1128/iai.62.10.4515-4525.1994.