PMID- 7927880
OWN - NLM
STAT- MEDLINE
DCOM- 19941025
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 58
IP  - 6
DP  - 1994 Sep 15
TI  - Sulfonated aluminium phthalocyanines as sensitizers for photochemotherapy. 
      Effects of small light doses on localization, dye fluorescence and 
      photosensitivity in V79 cells.
PG  - 865-70
AB  - V79 cells incubated with di- or tetrasulfonated aluminium phthalocyanines (AlPcS2 
      or AlPcS4) showed a granular fluorescence pattern. Co-staining with the 
      lysosomotropic dye acridine orange (AO) indicated that the granules that were 
      stained by these photoactive phthalocyanines were identical to lysosomes. Small 
      light exposures made the lysosomes permeable to the dyes without inactivating the 
      cells. Also, the lysosomal enzymes beta-AGA and cathepsin (L+B) were inactivated 
      by small light exposures when AlPcS4 was present. Such small and almost nontoxic 
      light exposures caused a redistribution of the dyes in the cells that was 
      accompanied by a more than 10-fold increase in the fluorescence quantum yields of 
      the dyes. Surprisingly, this redistribution and increase in fluorescence did not 
      result in any significant increase in the photosensitivity of the cells.
FAU - Moan, J
AU  - Moan J
AD  - Department of Biophysics, Institute for Cancer Research, Oslo, Norway.
FAU - Berg, K
AU  - Berg K
FAU - Anholt, H
AU  - Anholt H
FAU - Madslien, K
AU  - Madslien K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 0 (Organometallic Compounds)
RN  - 0 (Photosensitizing Agents)
RN  - 122170-90-5 (aluminum tetrasulfophthalocyanine)
RN  - 68637-19-4 (aluminum phthalocyanine disulfonate)
RN  - EC 3.2.1.52 (Acetylglucosaminidase)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.1 (Cathepsin B)
RN  - EC 3.4.22.15 (Cathepsin L)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acetylglucosaminidase/analysis
MH  - Acridine Orange/analysis/pharmacokinetics
MH  - Animals
MH  - Cathepsin B/analysis
MH  - Cathepsin L
MH  - Cathepsins/analysis
MH  - Cells, Cultured
MH  - Cricetinae
MH  - Cricetulus
MH  - Cysteine Endopeptidases
MH  - *Endopeptidases
MH  - Fibroblasts/*drug effects/*metabolism
MH  - Fluorescent Dyes/analysis/*pharmacokinetics/*pharmacology
MH  - Indoles/*pharmacokinetics/*pharmacology
MH  - Light
MH  - Lysosomes/drug effects/enzymology/metabolism
MH  - Organometallic Compounds/*pharmacokinetics/*pharmacology
MH  - *Photochemotherapy
MH  - Photosensitizing Agents/*pharmacokinetics/*pharmacology
MH  - Spectrometry, Fluorescence
EDAT- 1994/09/15 00:00
MHDA- 1994/09/15 00:01
CRDT- 1994/09/15 00:00
PHST- 1994/09/15 00:00 [pubmed]
PHST- 1994/09/15 00:01 [medline]
PHST- 1994/09/15 00:00 [entrez]
AID - 10.1002/ijc.2910580620 [doi]
PST - ppublish
SO  - Int J Cancer. 1994 Sep 15;58(6):865-70. doi: 10.1002/ijc.2910580620.