PMID- 7928184
OWN - NLM
STAT- MEDLINE
DCOM- 19941115
LR  - 20100324
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 35
IP  - 11
DP  - 1994 Oct
TI  - Expression of multiple cytokines and IL-1RA in the uvea and retina during 
      endotoxin-induced uveitis in the rat.
PG  - 3873-83
AB  - PURPOSE: Ample evidence is available demonstrating that cytokines play a pivotal 
      role in the pathogenesis of uveitis. Because little is known concerning the site 
      of cytokine synthesis in the eye, cytokine mRNA expression was analyzed in the 
      uvea, retina, and cornea during endotoxin-induced uveitis (EIU) in the rat. 
      METHODS: RNA was isolated from the iris, ciliary body, choroid-sclera, retina, 
      and cornea at different points in time after foot-pad injection of 200 micrograms 
      lipopolysaccharide (LPS) in Lewis rats. Reverse-transcription polymerase chain 
      reaction analysis was used to determine tumor necrosis factor alpha (TNF-alpha), 
      interleukin 1 beta (IL-1 beta), IL-6, IL-10, interferon gamma (IFN-gamma), 
      monocyte chemotactic protein 1 (MCP-1), macrophage inflammatory protein 2 
      (MIP-2), and IL-1 receptor antagonist (IL-1RA) mRNA expression. RESULTS: Maximal 
      mRNA expression of all cytokines examined already was observed in the uvea 4 
      hours after systemic LPS injection, before the onset of clinical uveitis. 
      Elevated expression of TNF-alpha, IL-1 beta, IL-6, IFN-gamma, MCP-1, and MIP-2 
      was also observed concomitant with maximal uveitis, at 22 to 24 hours. Except for 
      IL-10 and IFN-gamma, all cytokines investigated were induced in the retina, with 
      maximal expression at 22 to 24 hours. Expression of IL-1RA was detected in the 
      uvea and retina at 4 hours and remained elevated up to 48 hours, when the 
      clinical uveitis started to decline. LPS did not induce cytokine expression in 
      the cornea. Strikingly, a considerable expression of IL-1RA was found in normal 
      corneas, suggesting an inherent control mechanism for IL-1-mediated responses. 
      CONCLUSIONS: Systemic LPS injection induces elevated mRNA expression of multiple 
      cytokines and IL-1RA in the uvea and retina during various stages of EIU. This 
      suggests that these mediators may contribute to the development and recovery of 
      this intraocular inflammation.
FAU - de Vos, A F
AU  - de Vos AF
AD  - Department of Ophthalmo-Immunology, The Netherlands Ophthalmic Research 
      Institute, Amsterdam.
FAU - Klaren, V N
AU  - Klaren VN
FAU - Kijlstra, A
AU  - Kijlstra A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Bacterial Toxins)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (Endotoxins)
RN  - 0 (Interleukin 1 Receptor Antagonist Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (salmonella toxin)
SB  - IM
MH  - Animals
MH  - Bacterial Toxins
MH  - Base Sequence
MH  - Cornea/immunology
MH  - Cytokines/*biosynthesis/genetics
MH  - DNA Primers
MH  - Disease Models, Animal
MH  - Endotoxins
MH  - Gene Expression
MH  - Interleukin 1 Receptor Antagonist Protein
MH  - Male
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Retina/*immunology
MH  - Salmonella
MH  - Sialoglycoproteins/*biosynthesis/genetics
MH  - Uvea/*immunology
MH  - Uveitis/*immunology
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1994 Oct;35(11):3873-83.