PMID- 7929187
OWN - NLM
STAT- MEDLINE
DCOM- 19941104
LR  - 20210212
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 269
IP  - 40
DP  - 1994 Oct 7
TI  - S-thiolation of glyceraldehyde-3-phosphate dehydrogenase induced by the 
      phagocytosis-associated respiratory burst in blood monocytes.
PG  - 25010-5
AB  - Chemical oxidants can induce the covalent binding of low molecular weight thiols 
      to reactive sulfhydryls on proteins (S-thiolation). We found that stimulation of 
      the respiratory burst of human blood monocytes resulted in S-thiolation of 
      several proteins, most prominently one of 38 kDa. This purified protein was 
      identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by enzyme 
      activity, immunoblotting, and amino acid analysis. After stimulation of the 
      respiratory burst, S-thiolation of GAPDH gradually increased, and cytosol GAPDH 
      activity decreased; so that at 60 min, GAPDH activity was reduced by 
      approximately 40%. Activity was restored by the addition of the 
      sulfhydryl-reducing agent dithioerythritol. H2O2 appeared to be particularly 
      important in mediating S-thiolation during the respiratory burst. Exposure of 
      monocytes to H2O2 induced concentration-dependent S-thiolation of GAPDH and a 
      concomitant decrease in enzyme activity. The addition of respiratory burst 
      stimuli to lymphocytes, which lack a full respiratory burst, had no effect on 
      GAPDH S-thiolation or activity; but H2O2 induced S-thiolation of lymphocyte GAPDH 
      and inhibition of enzyme activity. Stimulation of monocytes from three patients 
      with chronic granulomatous disease resulted in no respiratory burst, S-thiolation 
      of GAPDH, or inactivation of GAPDH activity. The thiols covalently bound to 
      purified S-thiolated GAPDH were removed by dithioerythritol and were identified 
      as glutathione and cysteine; glutathione was predominant. These results indicate 
      that during the respiratory burst in monocytes, low molecular weight thiols can 
      bind to specific cytosolic proteins, including GAPDH. It is possible that 
      S-thiolation of cytosolic proteins serves to modulate cellular metabolic events 
      during phagocytosis.
FAU - Ravichandran, V
AU  - Ravichandran V
AD  - Department of Pediatrics (Immunology), Yale University School of Medicine, New 
      Haven, Connecticut 06520.
FAU - Seres, T
AU  - Seres T
FAU - Moriguchi, T
AU  - Moriguchi T
FAU - Thomas, J A
AU  - Thomas JA
FAU - Johnston, R B Jr
AU  - Johnston RB Jr
LA  - eng
GR  - AI 24782/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Sulfhydryl Compounds)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - O3C74ACM9V (Ethylmaleimide)
SB  - IM
MH  - Ethylmaleimide/pharmacology
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*metabolism
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - Monocytes/*metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - *Phagocytosis
MH  - *Respiratory Burst
MH  - Sulfhydryl Compounds/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1994/10/07 00:00
MHDA- 2001/03/28 10:01
CRDT- 1994/10/07 00:00
PHST- 1994/10/07 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1994/10/07 00:00 [entrez]
AID - S0021-9258(17)31491-6 [pii]
PST - ppublish
SO  - J Biol Chem. 1994 Oct 7;269(40):25010-5.