PMID- 7929742
OWN - NLM
STAT- MEDLINE
DCOM- 19941115
LR  - 20190512
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 9
IP  - 5
DP  - 1994 May
TI  - Healthy deliveries from biopsied human embryos.
PG  - 912-6
AB  - Preimplantation genetic diagnosis was performed in 122 embryos obtained by IVF 
      from 11 patients carriers of haemophilia, Duchenne's muscular dystrophy, Barth's 
      syndrome, cystic fibrosis, Pelizaeus-Merzbacher syndrome or Rett's syndrome. 
      After multiplex polymerase chain reaction (PCR) or fluorescence in situ 
      hybridization (FISH) analysis with multiple probes, 28 embryos diagnosed as not 
      affected were replaced. Of these, eight implanted (28%) and produced three 
      ongoing pregnancies, three deliveries of four babies and a biochemical pregnancy. 
      However, one case screened for cystic fibrosis was misdiagnosed and the pregnancy 
      was terminated. In order to evaluate the efficiency of multiplex PCR, 55 
      non-replaced embryos were reassessed by PCR or by FISH. Identical results were 
      obtained in all cases. However, one embryo which had only X-chromosome specific 
      amplification by PCR was found to be XO in all its cells by FISH. Although 
      multiplex PCR is demonstrated to be reliable for sexing of human embryos, FISH 
      has the additional advantages of supplying ploidy assessment while not being 
      affected by contamination.
FAU - Grifo, J A
AU  - Grifo JA
AD  - Department of Obstetrics and Gynecology, New York Hospital-Cornell University 
      Medical Center, NY 10021.
FAU - Tang, Y X
AU  - Tang YX
FAU - Munne, S
AU  - Munne S
FAU - Alikani, M
AU  - Alikani M
FAU - Cohen, J
AU  - Cohen J
FAU - Rosenwaks, Z
AU  - Rosenwaks Z
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Biopsy/adverse effects/*methods
MH  - Blastomeres/cytology
MH  - Diagnostic Errors
MH  - *Embryo, Mammalian/cytology
MH  - Female
MH  - Fertilization in Vitro
MH  - Genetic Diseases, Inborn/*diagnosis/genetics
MH  - Heterozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant, Newborn
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Prenatal Diagnosis/adverse effects/*methods
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a138616 [doi]
PST - ppublish
SO  - Hum Reprod. 1994 May;9(5):912-6. doi: 10.1093/oxfordjournals.humrep.a138616.